We narrowed to 18,591 results for: IRE
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Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO human shRNA 2 c-MAF
Plasmid#29433DepositorInsertc-MAF shRNA 2 (MAF Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTn
Plasmid#154369PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
mr in pUASP
Plasmid#112981PurposeP element vector for transposon insertion of mr (APC2) into Drosophila genomeDepositorInsertmr (APC2) (mr Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO1
Plasmid#55765PurposeEncoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cellsDepositorInsertY-GECO1.0
UseLab constructedTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K395A/K396A
Plasmid#162656PurposeExpresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K395 and K396 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-CT
Plasmid#18069DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-N43H
Plasmid#82344PurposeExpresses FLAG-tagged SLC30A10-N43H in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationN43HPromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
UseTagsmRFPExpressionPlantMutationPromoterRD21A nativeAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-D248A
Plasmid#82346PurposeExpresses FLAG-tagged SLC30A10-D248A in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationD248APromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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XE158 GFP-delta LZ-XDpr-CS2+
Plasmid#16764DepositorInsertdelta LZ-XDpr (dact1-b Frog)
UseXenopus expressionTagsgfpExpressionMutationXDpr is truncated to delete the first 129 amino a…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWL502-PAM
Plasmid#174382PurposePlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterraneiDepositorInsertPAM-spacer
UseCRISPR; Archaeal expressionTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-F63Q
Plasmid#104363PurposeExpresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationmutated the following amino acid: F63QPromoterCMVAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Barcode backbone
Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3409
Plasmid#179574PurposePceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPceh-12Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
UseTagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MPC11 IgG2b gene
Plasmid#127248PurposeExpresses intact IgG2b gene in mammalian cellsDepositorInsertIgG2b gene with exons and alt pAs (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only