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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al bioRxiv 2023.09.08.556841

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHel3

Plasmid

#102961

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Article

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Shuttle vector

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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NSP6-Flag

Plasmid

#210345

Purpose

Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBA-mCherry-EGFP-PIM

Plasmid

#111758

Purpose

Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

chicken beta-actin

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.5

Plasmid

#212523

Purpose

Encodes SARS-CoV-2 variant XBB.1.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SmBiT

Plasmid

#223618

Purpose

Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pKC-4.04

Plasmid

#112085

Purpose

Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

Depositor

James Inglese

Article

Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

Insert

3XFLAG firefly luciferase-beta-globin (HBB Photinus pyralis, Human, Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEX-PK-hLC3

Plasmid

#61458

Purpose

Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3 (MAP1LC3B Fission Yeast, Human)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Mic60

Plasmid

#191980

Purpose

Expression of V5-tagged Mic60, a component of the MICOS complex.

Depositor

Luca Scorrano

Insert

Mic60-V5

Use

Retroviral

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E

Plasmid

#212657

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-Q63L

Plasmid

#12968

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA constitutively active (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Anti-Mitofusin-1 [N111/24R]

Plasmid

#140072

Purpose

Mammalian Expression Plasmid of anti-Mitofusin-1 (Mouse). Derived from hybridoma N111/24.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mitofusin-1 (Mouse) recombinant mouse monoclonal antibody (Mfn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g7

Plasmid

#80790

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST EGFP-flag-CAD

Plasmid

#188118

Purpose

Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

EGFP tag and Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-EF1α-H2B-HaloTag-IRES-puro

Plasmid

#247460

Purpose

Piggybac based mammalian expression vector for histone H2B HaloTag, driven by the EF1a promoter.

Depositor

Kazuhiro Maeshima

Article

Hibino et al Nat Commun. 2024 Aug 21;15(1):7152. doi:

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQmod2S-GG

Plasmid

#191346

Purpose

Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

Plasmid

#185910

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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