Skip to main content
Addgene
Showing: 8701 - 8720 of 157954 results

  2. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

    Plasmid
    #154987
    Purpose
    Lentiviral vector expressing Human ACE2 and TMPRSS2
    Depositor
    Sonja Best
    Insert
    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154987-plasmid-map-sequence-id-310100

    Enlarge

  7. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Use
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

    Enlarge

  11. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid
    #139990
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VQR=D1135V/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139990-plasmid-map-sequence-id-275521

    Enlarge

  14. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid
    #205703
    Purpose
    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines
    Depositor
    Kai Johnsson
    Article
    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.
    Insert
    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    205703-plasmid-map-sequence-id-423137

    Enlarge

  16. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid
    #66932
    Purpose
    Mammalian Expression of TRAF2
    Depositor
    Michael Karin
    Insert
    TRAF2 (TRAF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66932-plasmid-map-sequence-id-190248

    Enlarge

  19. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid
    #197504
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197504-plasmid-map-sequence-id-386610

    Enlarge

Showing: 8701 - 8720 of 157954 results