We narrowed to 18,867 results for: IRE;

1. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78307

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADflluw
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIPZ-DoxOn-shCUX1-5150

   Plasmid

   #90469

   Purpose

   Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

   Depositor

   Alain Nepveu

   Article

   Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

   Insert

   TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-Rap2A-3xFLAG

   Plasmid

   #87973

   Purpose

   Expression of Rap2A in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_SPIC_P2A_Hygro_Barcode

   Plasmid

   #120484

   Purpose

   Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPIC (SPIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cdk6-DN

   Plasmid

   #1867

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk6 (CDK6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dominant negative, D to N in conserved KLAD*FGLAR…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRG199-bmp4

   Plasmid

   #99076

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Bmp4 (also known as bmp) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEE495

   Plasmid

   #149291

   Purpose

   T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLENTI CMV GFP-TREX1 V235fs BLAST

    Plasmid

    #164236

    Purpose

    lentiviral plasmid for GFP-TREX1 V235fs

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V235 frame shift mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

    Plasmid

    #169369

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CRISPR V2 sgMTFMT_1

    Plasmid

    #106318

    Purpose

    Express Cas9 and sgRNA targeting MTFMT

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTFMT
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FN207

    Plasmid

    #50497

    Depositor

    Kenneth Yamada

    Article

    Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

    Insert

    NM_001306129.1 (FN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    repeats 6-10

    Promoter

    T7

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMXs-IP-EGFP-ULK1(1-828)

    Plasmid

    #38198

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 829-1051

    Promoter

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAsCpf1GG

    Plasmid

    #131011

    Purpose

    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of AsCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL106P-LUC-ENTR1A(w451-1)

    Plasmid

    #17455

    Purpose

    Gateway entry vector, Degron N-term Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    FKBP-Luciferase fusion (MTOR firefly)
    

    Use

    Luciferase; Entry vector

    Tags

    FKBP12DD

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIPZ shCUX1-5328

    Plasmid

    #100815

    Purpose

    Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

    Depositor

    Alain Nepveu

    Article

    Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

    Insert

    TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge