176,130 results

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pCMV GFP1-9

Plasmid

#182244

Purpose

Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

Depositor

Stéphanie Cabantous

Publication

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP 1-9 OPT

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LXRE_Luc

Plasmid

#177622

Purpose

synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

Depositor

Thomas Burris

Publication

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

3X LXRE

Use

Luciferase

Expression

Mammalian

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Publication

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Publication

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Publication

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Publication

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1 and AAV9

Insert

GFP-fGFP

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Publication

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CARPID dCasRx-BASU

Plasmid

#153303

Purpose

express CARPID dCasRx-BASU fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Publication

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

dCasRx-BASU

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Publication

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mNeonGreen-Rab7

Plasmid

#129603

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-ASAP3Kv-WPRE

Plasmid

#132332

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Publication

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3Kv

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Publication

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDisplay-mgSrtA

Plasmid

#125792

Purpose

Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

Depositor

P. Chen

Publication

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

mgSrtA

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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