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pAG426GAL-HHV-3_PR

Plasmid

#203505

Purpose

Expresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HHV-3 protease domain

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-NWV_3CL

Plasmid

#203470

Purpose

Expresses NWV 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

NWV 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HTLV_PR

Plasmid

#203467

Purpose

Expresses HTLV protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HTLV protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AdoLightG

Plasmid

#187465

Purpose

Expresses green adenosine indicator AdoLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AdoLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-PV_3C

Plasmid

#203472

Purpose

Expresses PV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

PV 3C protease (PVgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-SV-A_3C

Plasmid

#203474

Purpose

Expresses SV-A 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SV-A 3C protease (Hk1_gp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-1x-HIV-2_PR

Plasmid

#201935

Purpose

Expresses HIV-2 protease from a GAL-1x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-2 protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Protein3

Plasmid

#168923

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Protein3 (HCNV63gp3 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-Spike

Plasmid

#168891

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-Spike (S )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-In18runon-PTPN22

Plasmid

#195377

Purpose

A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Gateway donor vector

Mutation

Intron 18-runon PTPN22

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-In18runon-PTPN22

Plasmid

#195379

Purpose

Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Intron 18-runon PTPN22

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Ex1-17-PTPN22

Plasmid

#195375

Purpose

Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Tags

FLAG

Expression

Mammalian

Mutation

PTPN22 mutant containing exon 1 to 17

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2-pET151

Plasmid

#177870

Purpose

Expresses mouse Talin 1 F2 domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 domain (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-pET151

Plasmid

#177877

Purpose

Expresses mouse Talin 1 F2F3 domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 domain (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

Plasmid

#186414

Purpose

AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli)

Use

Gateway destination vector

Tags

Nuclear localization signal

Promoter

Basal promoter of the FOG gene

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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