Addgene

We narrowed to 11,032 results for: Hal

Showing: 1721 - 1760 of 11032 results

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pEXP(V5_H2B_APEX2)

Plasmid

#107597

Purpose

Expresses V5_H2B_APEX2 in mammallian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

H2B-APEX2

Use

Tags

H2B and V5

Expression

Mammalian

Mutation

Promoter

CMV Dox inducible

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T17N

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
Venus Cam KII alpha

Plasmid

#29428

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pCDZ2FE_Champ1_1-812

Plasmid

#105675

Purpose

Expresses Flag-tagged full-length human Champ1 protein

Depositor

Thanos Halazonetis

Insert

Champ1 (CHAMP1 Human)

Use

Tags

2X Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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57F03_LexA-GAD

Plasmid

#83256

Purpose

Expresses LexA-GAD under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 L61

Plasmid

#15903

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGL4.11 promPDE4D3 Ebox mut

Plasmid

#27304

Depositor

David E. Fisher

Article

Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

Insert

prom PDE4D3 (PDE4D Human)

Use

Luciferase

Tags

Expression

Mutation

Ebox CACATG changed to GAGATG

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pZL1-ZF - myelin basic protein a (mbpa)

Plasmid

#103007

Purpose

Plasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.

Depositor

Marnie Halpern

Article

Brosamle et al Glia. 2002 Jul;39(1):47-57.

Insert

myelin basic protein (mbpa Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Sp6

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pGEX4T3-NL2CT

Plasmid

#163623

Purpose

mouse NL2 N-terminally tagged with GST

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse NL2 (Nlgn2 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcA17/F527

Plasmid

#17684

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S17A Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 17 changed to Ala. Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228657

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

SLEV portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228653

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

MVEV portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTbp-Firefly-Chantico

Plasmid

#177836

Purpose

Luciferase reporter for mouse Tbp with Chantico promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Tbp

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-Chantico

Plasmid

#163897

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-ocitnahC

Plasmid

#163898

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter in reverse orientation

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAEL017410 MIP R

Plasmid

#115411

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

CCHamide-2 receptor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL008296 CCAP R

Plasmid

#115402

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

ovo isoform X1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL017414 TK R

Plasmid

#115396

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

phenoloxidase 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Rab33bb cds in pGem-T Easy

Plasmid

#80524

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab33bb

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcAla12/Ala17

Plasmid

#17682

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A S17A (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Ser 17 changed to Ala.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcA12/F527

Plasmid

#17683

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
PX458_RAD21_1

Plasmid

#64057

Purpose

Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_CREB1_1

Plasmid

#64939

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5myc POSH

Plasmid

#15908

Depositor

Alan Hall

Article

Tapon et al EMBO J. 1998 Mar 2. 17(5):1395-404.

Insert

POSH (Sh3rf1 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE1-HTR5p

Plasmid

#210542

Purpose

Cloning A. thaliana HTR5 promoter as GreenGate module E1

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pHTR5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CEBPB_1

Plasmid

#64036

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CEBPB_2

Plasmid

#64047

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4 TNRC6A

Plasmid

#20021

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Article

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Use

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T Sam68

Plasmid

#17687

Depositor

David Shalloway

Article

Resnick et al Oncogene. 1997 Sep;15(11):1247-53.

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1721 - 1760 of 11032 results

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