We narrowed to 8,905 results for: Ott
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Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
ExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B
Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Human, Synthetic)
UseMultisite gatewayMutationhuman amino acid sequence but different codon usa…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinHFDD
Plasmid#134462PurposeM1 SpastinHFDD expressionDepositorInsertM1 Spastin (SPAST Human)
ExpressionMammalianMutationMet to Ala at amino acid 87; His to Asp at amino …PromoterCMVAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2049
Plasmid#79657PurposessAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple260-EmGFP WPRE
UseAAVPromoterPAX6 Retinal EnhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2045
Plasmid#79653PurposessAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple256-EmGFP WPRE
UseAAVPromoterPAX6 enhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2048
Plasmid#79656PurposessAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple259-EmGFP WPRE
UseAAVPromoterPAX6 MiniPromoterAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterMET17Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2043
Plasmid#79651PurposessAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple254-EmGFP WPRE
UseAAVPromoterPAX6 Retinal EnhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)
Plasmid#214889PurposeExpresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanExpressionWormAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)
Plasmid#214890PurposeExpresses the optogenetic tool BiPOLES in BWMs of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanExpressionWormAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB17 (punc-17::QuasAr)
Plasmid#214888PurposeExpresses the voltage sensor QuasAr2 in cholinergic neurons of C. elegans.DepositorInsertQuasAr2
ExpressionWormAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)
Plasmid#214891PurposeExpresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 huPQBP1
Plasmid#217449PurposeExpresses full-length human PQBP1 from a His-SUMO bacterial expression vectorDepositorInsertPQBP1 (PQBP1 Human)
ExpressionBacterialAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA E45A
Plasmid#217438PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).DepositorInsertgag/pol
UseLentiviralMutationCA E45AAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA M66I
Plasmid#217440PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).DepositorInsertgag/pol
UseLentiviralMutationCA M66IAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A
Plasmid#204622PurposeSingle-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.DepositorInsertmRFP1
ExpressionMammalianPromoterEF1aAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
phABCA3-eGFP_CRISPR_Donor
Plasmid#188540PurposeHomologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locusDepositorInsertEGFP
UseCRISPR; Donor for homologous recombinationAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-SENP8 sg2R-P2A-Hygro
Plasmid#124300PurposeLentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertSENP8 (SENP8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2046
Plasmid#79654PurposessAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple257-EmGFP WPRE
UseAAVPromoterPAX6 EnhancerAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1930
Plasmid#79779PurposeHigh copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insertDepositorInsertPle257
UsePleiades promoter project [sic, pleaides plieades]PromoterPle257 (PAX6 MiniPromoter)Available SinceNov. 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2047
Plasmid#79655PurposessAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple258-EmGFP WPRE
UseAAVPromoterPAX6 EnhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1931
Plasmid#79780PurposeHigh copy number pUC vector containing Ple258 (PAX6 MiniPromoter) insertDepositorInsertPle258
UsePleiades promoter project [sic, pleaides plieades]PromoterPle258 (PAX6 MiniPromoter)Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1929
Plasmid#79778PurposeHigh copy number pUC vector containing Ple256 (PAX6 MiniPromoter) insertDepositorInsertPle256
UsePleiades promoter project [sic, pleaides plieades]PromoterPle256 (PAX6 MiniPromoter)Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1928
Plasmid#79777PurposeHigh copy number pUC vector containing Ple255 (PAX6 MiniPromoter) insertDepositorInsertPle255
UsePleiades promoter project [sic, pleaides plieades]PromoterPle255 (PAX6 MiniPromoter)Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1927
Plasmid#79776PurposeHigh copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insertDepositorInsertPle254
UsePleiades promoter project [sic, pleaides plieades]PromoterPle254 (PAX6 MiniPromoter)Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB2E
Plasmid#195288PurposeBicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK EGFP 2A Puro
ExpressionMammalianPromoterEFSAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorAvailable SinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-Rheb (Q64L)-pcw107
Plasmid#64607Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVSV-G
Plasmid#138479PurposeExpresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.DepositorInsertVSV-G envelop protein
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-MDFIC
Plasmid#207835PurposeTagged MDFIC for western blotting and imagingDepositorAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q67H/N74D
Plasmid#217441PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).DepositorInsertgag/pol
UseLentiviralMutationCA Q67H,N74DAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB2D
Plasmid#195286PurposeBicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selectionDepositorInsertDTR EGFP 2A Puro
ExpressionMammalianPromoterEFSAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Mkk7-JNK2 fusion-pcw107
Plasmid#64618PurposeMkk7-JNK2 fusion - barcoded activating construct of JNK pathwayDepositorInsertMap2k7 linker MAPK9
UseLentiviralTagsFLAGMutationmouse-human fusionPromoterPGKAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
psd44-iGFP-MFGE8-long
Plasmid#46847Purposeexpression of MFGE8 in mammalian cellsDepositorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 WT-Puro
Plasmid#123319PurposeLentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoterDepositorAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-WT_ABCA3-GFP
Plasmid#188548PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion proteinDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only