We narrowed to 4,010 results for: SPL
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Plasmid#190337PurposeExpressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertionDepositorInsertPE_CO-Mini-C, PCSK9 +1A insertion pegRNA
UseAAVAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST
Plasmid#194385PurposeGateway Destination vector for split TEV assaysDepositorInsertattR1-ORF-attR2-NTEV-TCS-GV-2xHA
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PE_CO-Mini-N
Plasmid#190336PurposeExpressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertionDepositorInsertPE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
UseAAVAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cGR2176
Plasmid#188258PurposePlasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcGR2176
UseCRISPRExpressionMammalianAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]
Plasmid#133328PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll F to S in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIND-HBSP
Plasmid#53113PurposeExpression of GAL4BD-tagged Hepatitis B splicing proteinDepositorInsertHBSP
TagsGAL4BDExpressionMammalianPromoterCMVAvailable SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH131
Plasmid#48260PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
GAL10/GAL1 bidirectional promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterGAL10/GAL1 bidirectionalAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]
Plasmid#133333PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll W to S in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-SmB'
Plasmid#1842DepositorAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHBS1410 GFP-TDP43 F-S
Plasmid#118799PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
IBMc071
Plasmid#161943PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmidDepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
ExpressionBacterialPromoterPBADAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-SmB
Plasmid#1841DepositorAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]
Plasmid#118804PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB6_EPB49
Plasmid#84015PurposeEPB49 minigene to test splicing eventDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-nTEVp
Plasmid#179666PurposeRas Binding Domain split-nTEVPDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-cTEVp
Plasmid#179667PurposeRas Binding Domain split-cTEVPDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB6_ATR
Plasmid#84016PurposeATR minigene to test splicing eventDepositorAvailable SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only