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pJG/ Inducible ALPHA MHC

Plasmid

#55792

Purpose

transgene expression driven by inducible ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

Type

Empty backbone

Expression

Mammalian

Promoter

Inducible ALPHA MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNC-Cam1304-MCS

Plasmid

#193387

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCM4.4

Plasmid

#217648

Purpose

an ermE*p-cas9-driven expression, as an alternative plasmid

Depositor

Huimin Zhao

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

Codon Optimized SpCas9

Use

CRISPR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Expression

Bacterial

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAL-pck_T

Plasmid

#74068

Purpose

pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (T)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pYPQ134-tRNA

Plasmid

#179213

Purpose

Golden Gate entry vector; 4th gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

no promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src-myc

Plasmid

#25933

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-Src (Src Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDM344

Plasmid

#108974

Purpose

shuttle vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAL-pck_NT

Plasmid

#74069

Purpose

pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (NT)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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