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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Publication

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-iRFP670

Plasmid

#99377

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-iRFP670

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway Cloning

Mutation

V617F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-mFlagEts1

Plasmid

#86099

Purpose

expresses flag tagged Ets1 in mammalian cells

Depositor

Barbara Graves

Publication

Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

Insert

Ets1 (Ets1 Mouse)

Tags

Flag tag; kanamycin selection

Expression

Mammalian

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p5E-gfap

Plasmid

#82401

Purpose

Gateway-compatible zebrafish gfap promoter

Depositor

Judith Eisen

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

gfap promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Publication

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_NS3_Flag

Plasmid

#79635

Purpose

Third generation Self-inactivating lentivector expressing NS3 from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS3

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJQ200SK

Plasmid

#78497

Purpose

Suicide vector for gene replacement

Depositor

Michael Hynes

Publication

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide plasmid

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-HA p21

Plasmid

#78782

Purpose

To overexpress p21 in Mammalian Cell

Depositor

Jaewhan Song

Publication

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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