12,598 results

1. pLVX-Puro-mGSDME

   Plasmid

   #154877

   Purpose

   Expresses mGSDME in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

   Plasmid

   #209080

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. M_ST1n_VPR

   Plasmid

   #63799

   Purpose

   ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   ST1-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMSCVpuro-Gcn5

   Plasmid

   #63706

   Purpose

   retroviral expression of mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PBGFAP-eGFP

   Plasmid

   #40975

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Insert

   Mouse GFAP promoter fragment
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEFmEGFP-mDia2

   Plasmid

   #25407

   Depositor

   Arthur Alberts

   Article

   Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

   Insert

   mDia2 (Diaph3 Mouse)
   

   Use

   Tags

   EGFP, His, and Myc

   Expression

   Mammalian

   Mutation

   Encodes mDia2

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RSV-Flag-Brd4

   Plasmid

   #86616

   Purpose

   Flag tagged mouse Brd4 expression construct for cell transfection

   Depositor

   Mario García-Domínguez

   Article

   Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

   Insert

   Brd4 (Brd4 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28b_RanT24N

   Plasmid

   #186271

   Purpose

   Expression vector of His-RanT24N in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged and His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed T24 into N

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TMC1-TwinStrepII

   Plasmid

   #83267

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TMC1 (Tmc1 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. WT NFAT1

    Plasmid

    #11100

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RSV-Flag-Brd2

    Plasmid

    #86614

    Purpose

    Flag tagged mouse Brd2 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd2 (Brd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCh-KIF1A*-strep

    Plasmid

    #120165

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 1A (Kif1a Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF1A: 1-405 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sAB-K29

    Plasmid

    #204735

    Purpose

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

    Depositor

    Minglei Zhao

    Article

    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

    Insert

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-Mycn-IRES-GFP

    Plasmid

    #35394

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

    Inserts

    Mycn (Mycn Mouse)
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-EGFP-p50

    Plasmid

    #107977

    Purpose

    Expresssion of NF-kB p50

    Depositor

    Georg Weber

    Insert

    NF-kB p50 (Nfkb1 Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Amino acids VQACI added to C-terminus

    Promoter

    CMV

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge