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Addgene

We narrowed to 943 results for: fp

Showing: 881 - 900 of 943 results
  1. PB-CAG::Nucbow

    Plasmid
    #158996
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. Tol2-CAG::Nucbow

    Plasmid
    #158992
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pQFmcs-2H12.D11-scarREF

    Plasmid
    #221184
    Purpose
    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)
    Depositor
    Inserts
    cdGreen2
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen2) and PQ5 (cumate-inducible)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. ROSA26(MultiFPsΔPuro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Insert
    2xFKBP-smFP(myc)-KDEL
    Use
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. LSB-hsa-miR-149-5p

    Plasmid
    #103255
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-149-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 FPs. . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Insert
    hsa-miR-149-5p target (MIR149 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 881 - 900 of 943 results