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Green Glifon600

Plasmid

#126207

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon600

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Expression

Mammalian

Mutation

D210N, catalytically inactive

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJ3M-Mst1 K59R

Plasmid

#12204

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst1 (STK4, MST1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive: K59R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-UTY-F

Plasmid

#17439

Purpose

Expression of UTY in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTY (UTY Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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DRH313: FCK-CheRiff-eGFP

Plasmid

#51693

Purpose

Channelrhodopsin with improved sensitivity and minimal red light photocurrent

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2-EmGFP-mir9

Plasmid

#22741

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9

Expression

Mammalian

Available Since

Jan. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV4-p105

Plasmid

#23288

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p105 (NFKB1 Human)

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA4/BDD-FVIII

Plasmid

#41035

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

B domain deleted FVIII (F8 Human)

Expression

Mammalian

Mutation

B domain deletion

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Tags

His6-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
RIP-Timer (DM#285)

Plasmid

#15109

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

insulin II promoter (Ins2 Rat)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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