We narrowed to 24,357 results for: nes

1. pcDNA3-Venus-Venus-FLARE-Cameleon

   Plasmid

   #123351

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_EIF4E_eiF4E

   Plasmid

   #178474

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   EIF4E_eiF4E (EIF4E Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

   Plasmid

   #201831

   Purpose

   piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Inserts

   CAG
   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 PhLP1-myc-6xHis

   Plasmid

   #218176

   Purpose

   Expresses phosducin-like protein 1 in mammalian cells

   Depositor

   Barry Willardson

   Article

   Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

   Insert

   Phosducin like protein 1 (PDCL Human)
   

   Use

   Tags

   6x His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Use

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2xFLAGhYAP1-WW mutant

   Plasmid

   #17792

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 to Alanine; Proline 202 to Alanine

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1A hu_ncOGT WT

   Plasmid

   #154281

   Purpose

   Entry vector encoding non-tagged O-GlcNAc Transferase

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   WT OGT

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKD072

   Plasmid

   #136472

   Purpose

   SSB-C-term-GFP fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-C-term-GFP (ssb E. coli)
   

   Use

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP attached to C-terminus at Phe 178

   Promoter

   T7 promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_MAD2L1_HORMA

   Plasmid

   #178480

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   MAD2L1_HORMA (MAD2L1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM33_TNKS_TANK-peptide-bind

    Plasmid

    #178489

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    TNKS_TANK-peptide-bind (TNKS Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2xFLAGhYAP2-S127A

    Plasmid

    #17794

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Serine 127 to Alanine

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-ΔN-RNMT

    Plasmid

    #112708

    Purpose

    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG and HIV Rev nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120, mutated active-site D2…

    Promoter

    CMV

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SARS2-Spike-D614G

    Plasmid

    #166850

    Purpose

    For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2 virus)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pETM33_KPNA4_Arm

    Plasmid

    #178495

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KPNA4_Arm (KPNA4 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM33_KEAP1_KELCH

    Plasmid

    #178496

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KEAP1_KELCH (KEAP1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47870

    Purpose

    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mCherry-mCherry-FLARE-Cameleon

    Plasmid

    #123352

    Purpose

    Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_CEP55_EABR

    Plasmid

    #178469

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CEP55_EABR (CEP55 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM41_KEAP1_KELCH

    Plasmid

    #178476

    Purpose

    Bacterial expression of human domain with His-tag and MBP tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KEAP1_KELCH (KEAP1 Synthetic, Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM33_EPS15_EH

    Plasmid

    #178475

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    EPS15_EH (EPS15 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge