We narrowed to 1,949 results for: pCAG

Showing: 881 - 900 of 1949 results

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pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-dre

Plasmid

#124832

Purpose

Monomeric TagBFP2 and dre recombinase

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-dre

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-Citrine

Plasmid

#224565

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-Citrine

Use

CRISPR

Tags

T2A-Citrine

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-pac-T2A-dre

Plasmid

#124833

Purpose

Puromycin resistance gene and dre recombinase

Depositor

Mario Capecchi

Insert

pac-T2A-dre

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyB621-mCherry-HRasCT

Plasmid

#100282

Purpose

Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-621 aa) (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-621 aa)

Promoter

CAG promoter

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (K70A)

Plasmid

#41589

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

K70A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-nDsRedFL-miR9/9*/124

Plasmid

#177807

Purpose

The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*/124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP/RFP-int

Plasmid

#19818

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Type

Empty backbone

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Gag-FKBP-Myc

Plasmid

#177909

Purpose

HIV Gag-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

HIV Gag (gag )

Tags

FKBP12 and Myc tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-P834A

Plasmid

#188082

Purpose

Expresses human ATG9-P834A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

P834A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP1FBAR(516a.a.)-EGFP

Plasmid

#168505

Purpose

Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP1-FBAR (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-516

Promoter

CAG

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bgh-GFP-Farpl

Plasmid

#115501

Purpose

expression of rat Farp1

Depositor

Thomas Biederer

Article

Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

Insert

Farp1 (Farp1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

chicken beta-actin

Available Since

Dec. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-FKO (NC-flag)

Plasmid

#159363

Purpose

Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS-CoV-2)

Tags

Flag and Flag

Expression

Mammalian

Mutation

Furin-cleavage site mutation

Promoter

CMV

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (NC-flag)

Plasmid

#156419

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase gRNA resistant

Plasmid

#163411

Purpose

Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted. gRNA-t…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only