19,824 results

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pCMVβ-p300.DY-myc

Plasmid

#30490

Depositor

Tso-Pang Yao

Insert

p300 (EP300 Human)

Tags

Myc

Expression

Mammalian

Mutation

aspartic acid 1399 converted to tyrosine (Acetyla…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223_HSP90B1_WT

Plasmid

#82130

Purpose

Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSP90B1 (HSP90B1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-IL-1beta-mNeonGreen

Plasmid

#166783

Purpose

FRET analysis (pro-IL-1beta cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-IL-1b-mNeongreen (IL1B Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-cyto

Plasmid

#113917

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pU6d:sgRNA#5

Plasmid

#64249

Purpose

expresses sgRNA under U6d promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ER beta

Plasmid

#28237

Depositor

Michael Mancini

Insert

ER beta (ESR2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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7TG

Plasmid

#24314

Depositor

Roel Nusse

Article

Brugmann et al Development. 2007 Sep . 134(18):3283-95.

Insert

7xTcf-eGFP

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 reporter

Plasmid

#91704

Purpose

lentiviral luciferase reporter containing CDH1 promoter

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CDH1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1

Plasmid

#65807

Purpose

expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PAB1 (PABPC1 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP (CF)

Plasmid

#20160

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

CFP-FKBP (FKBP1A Human)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA TrkB

Plasmid

#24088

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkB (Ntrk2 Rat)

Expression

Mammalian

Mutation

R133I

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Raichu-RhoA-CR

Plasmid

#40258

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Raichu-RhoA-CR (RHOA Human, Synthetic)

Expression

Mammalian

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SZT2

Plasmid

#87035

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Promoter

EF1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAP1_WT_V5

Plasmid

#82994

Purpose

Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TRAP1 (TRAP1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-mir30

Plasmid

#14758

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

mir30 cassette

Use

RNAi

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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