159,617 results

1. pHAGE-RAD50

   Plasmid

   #116784

   Purpose

   Lentiviral expression of RAD50

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   RAD50 (RAD50 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1.sh.beta-catenin.1248

   Plasmid

   #19761

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   small hairpin RNA against beta-catenin (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. hyeA3A-BE4max

   Plasmid

   #157944

   Purpose

   Mammalian expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   hyeA3A-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGB 35S:Renilla:Tnos (GB0109)

   Plasmid

   #68215

   Purpose

   Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Renilla
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-eGFP-2A-mCherry_D4H

   Plasmid

   #194875

   Purpose

   Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-D4H
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. IGF2BP1

   Plasmid

   #155676

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   IGF2BP1 (IGF2BP1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_221

   Plasmid

   #169142

   Purpose

   Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

   Depositor

   David Root, John Doench

   Insert

   EGFP , EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-Zeo

    Plasmid

    #75088

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-Arl13b-mCardinal

    Plasmid

    #194542

    Purpose

    Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-GATA4

    Plasmid

    #192896

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Codon-optimized

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iCAP-ROOT

    Plasmid

    #192262

    Purpose

    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

    Depositor

    Li Ye

    Article

    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

    Insert

    Synthetic construct for ROOT VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP3545

    Plasmid

    #46888

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4-Chk1-Flag

    Plasmid

    #22894

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Chk1 (CHEK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPG

    Plasmid

    #71248

    Purpose

    improved luciferase reporter gene plasmid

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lifeact-Halo

    Plasmid

    #193328

    Purpose

    mammalian transient expression - expression of Lifeact-Halo

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge