We narrowed to 12,197 results for: 112

1. pFUW-TetO-Txnip

   Plasmid

   #178437

   Purpose

   doxycycline-inducible expression of Mouse Txnip in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   Txnip (Txnip Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMal-SLFN2

   Plasmid

   #174322

   Purpose

   Bacterial expression of MBP-tagged mouse SLFN2

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   SLFN2 (Slfn2 Mouse)
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS540-pCSDest2-AcrIIC4Hpa

   Plasmid

   #113434

   Purpose

   Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS542-pCSDest2-AcrIIC5Smu

   Plasmid

   #113435

   Purpose

   Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPT-ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49229

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. px335-sgRNA-EF(Cas9n)-Eif4a1-R

   Plasmid

   #122345

   Purpose

   Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Eif4a1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hisSUMO-Gli1

   Plasmid

   #87225

   Purpose

   Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   GLI1 (Gli1 Mouse)
   

   Tags

   hisSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs_FLAG-FSF1

   Plasmid

   #110641

   Purpose

   Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   FSF1 (FSF1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQTEV-AK1

   Plasmid

   #31290

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   adenylate kinase 1 (AK1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

    Plasmid

    #67648

    Purpose

    I-SceI cleavage site at lys1-hphMX6

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    lys1 (lys1 Fission Yeast)
    hphMX6
    

    Use

    To test i-scei cleavage efficiency

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS315-48nt-telo-I-SceI

    Plasmid

    #67623

    Purpose

    telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    3' gal1 unique sequence
    ura4 (ura4 Fission Yeast)
    48 bp telomere repeats
    hph
    

    Use

    Yeast cen/ars plasmid

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMflT-o4

    Plasmid

    #101312

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Q842gp120-8his

    Plasmid

    #100932

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. DT050A

    Plasmid

    #159411

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23101 promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1180 EACMCV DNA-B 1.6mer

    Plasmid

    #159137

    Purpose

    Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-B

    Depositor

    Vincent Fondong

    Article

    Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

    Insert

    ACMV DNA-B 1.5mer
    

    Use

    Infectious clone for bombarding plants

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFL-SV40-GNB2 5'-UTR

    Plasmid

    #115355

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

    Depositor

    Ming-Chih Lai

    Article

    Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

    Insert

    GNB2 5'-UTR (GNB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49218

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX2T AtFC1

    Plasmid

    #136363

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana ferrochelatase1

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    Full-length cDNA of Arabidopsis thaliana ferrochelatase1 (FC1 Mustard Weed)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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