We narrowed to 12,359 results for: NSI

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pOmnibac_ZZ_linker_K560_GFP_SNAP

Plasmid

#159720

Purpose

Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (only amino acids 1-560), K560 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Mutation

Only amino acids 1-560

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A ΔWIR

Plasmid

#215507

Purpose

Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

Plasmid

#180644

Purpose

Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

ULK1 (ULK1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

Residues 833-1050

Promoter

pCAG

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

Plasmid

#137143

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K120R/K164R)

Plasmid

#72556

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K120R/K164R

Promoter

E1B minimal promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV2 TEV-NSP12

Plasmid

#154410

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-NSP5

Plasmid

#154404

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-S

Plasmid

#154415

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of s…

Mutation

Many synonymous changes due to codon optimization

Available Since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 - myc ULK1 wt

Plasmid

#27623

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGL4.27_eSOX17.2

Plasmid

#195499

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Core distal endoderm enhancer region of SOX17 (SOX17 Human)

Tags

minP_luc2-hPEST

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-NSP1

Plasmid

#154400

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-SpCas9

Plasmid

#130968

Purpose

3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

3xHA-NLS-SpCas9

Use

CRISPR

Tags

3x HA, EGFP, and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO_FLAG.Ollas.VSVg_NGFR

Plasmid

#158324

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

FLAG.Ollas.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pLKO_S.FLAG.VSVg_NGFR

Plasmid

#158345

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.FLAG.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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