We narrowed to 26,411 results for: lars

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF102

Plasmid

#13236

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF102

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_GY11_00131271

Plasmid

#121355

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

GY11_00131271

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1R

Plasmid

#68236

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET-11a (GB1 WT - A34F)

Plasmid

#136609

Purpose

Expresses GB1 WT mutant (A34F)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT mutant (A34F)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E3E4 (630–1244)

Plasmid

#132628

Purpose

expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (630–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E4 (20–1244)

Plasmid

#132629

Purpose

expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)

Plasmid

#189793

Purpose

Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (Hbb Human, Mouse)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-mPlexinD1

Plasmid

#16424

Depositor

Jonathan Epstein

Article

Gitler et al Dev Cell. 2004 Jul . 7(1):107-16.

Insert

Plexin D1 (Plxnd1 Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pLNCX Myr His p110

Plasmid

#10836

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pBS-IT10

Plasmid

#66871

Purpose

Inntag 10: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 10

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRS405 VAC8-RFP

Plasmid

#166841

Purpose

Expresses Vac8-RFP in yeasts

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

RFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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YSBE 628 : pHYG_mCherry

Plasmid

#92092

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Tags

mCherry

Expression

Plant

Promoter

Ubiquitin10 promoter + 5' UTR

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFH85

Plasmid

#128198

Purpose

classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP216-Lenti-1.3CaMKIIa P-GFP-WPRE-pA

Plasmid

#62926

Purpose

pLenti vector backbone designed to express GFP from a 1.3CaMKIIa promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.3αCaMKII

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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