We narrowed to 5,868 results for: nls

1. pINO4-NLS-Pfv-Sapphire-HO

   Plasmid

   #203660

   Purpose

   Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   CRISPR

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTag-NLS-TagRFP-SV40

   Plasmid

   #117805

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-eGFP-NLS-RNF168

   Plasmid

   #133977

   Purpose

   mammalian expression vector of eGFP tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFUW-TetO-tdTomato-cNLS

   Plasmid

   #138532

   Purpose

   Lentiviral inducible expression of tdTomato with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-CMV

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-GFP-iLID-Ferritin

   Plasmid

   #227376

   Purpose

   Condensate module, light-inducible oligomerization 'core'

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   GFP 'core'
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG-GFP-TurboID-3XNLS

    Plasmid

    #209400

    Purpose

    Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-3XNLS
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-jAspSnFR3-mRuby3-NLS

    Plasmid

    #203494

    Purpose

    Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3-mRuby3-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear localization signal and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-BRX-eGFP-NLS

    Plasmid

    #217534

    Purpose

    AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    BMP reporter element and miniXon casette (Smad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BMP Reporter Element

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-NLS-BirA-2A-mCherry

    Plasmid

    #127781

    Purpose

    BirA construct in a PCI nls backbone driving mCherry activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    BirA
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-LacI-NLS-VP16

    Plasmid

    #103836

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-LacI-NLS-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

    Plasmid

    #121542

    Purpose

    Chronos with red nucleus-targeted fluorescence for excitatory neurons

    Depositor

    Matthias Prigge

    Insert

    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CamKII

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSSM16: pCMV-HA-NLS-SpuFz1

    Plasmid

    #205271

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SOX17-NLS-tdTomato-EPG

    Plasmid

    #210466

    Purpose

    Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

    Plasmid

    #124705

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E488Q)_NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-puro-NLS-LSSmKate2

    Plasmid

    #34586

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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