164,577 results

1. pHPHS232

   Plasmid

   #228356

   Purpose

   Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Inserts

   mTagBFP2
   Reverse tetracycline transactivator 3
   neo
   

   Use

   Tags

   Blasticidin S deaminase and iCasp9

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and gene trap

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. ChromosomesMembranesandCytoskeleton

   Plasmid

   #62450

   Purpose

   MXS_chaining vector with 4 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::H2B-TagBFPx3-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::mCherryx3-linker-betaActin-bGHpA
   CMV::Citrinex3-linker-alphaTubulin-bGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-hsRPA70 (MSW#473)

   Plasmid

   #208067

   Purpose

   Expression of GFP-tagged hsRPA1 in human cells

   Depositor

   Marc Wold

   Article

   Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

   Insert

   RPA1 (RPA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. KillerRed-LacI-NLS

   Plasmid

   #215102

   Purpose

   Expresses KillerRed-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   KillerRed-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCitrine-pBAD

   Plasmid

   #54723

   Purpose

   Localization: Protein Expression Vector , Excitation: 516, Emission: 529

   Depositor

   Michael Davidson, Robert Campbell, Roger Tsien, Oliver Griesbeck

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Use

   Tags

   6xHis and mCitrine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. iCAP-ROOT

   Plasmid

   #192262

   Purpose

   AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

   Depositor

   Li Ye

   Article

   Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

   Insert

   Synthetic construct for ROOT VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-T7gene1

   Plasmid

   #165505

   Purpose

   Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBad33_ISYmu1_reRNA

   Plasmid

   #205358

   Purpose

   Bacterial expression of ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISYmu1 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid

    #227273

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDAS12069_U6-pegRNA-mCherry

    Plasmid

    #177180

    Purpose

    pegRNA cloning plasmid with mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-PH-PLC-delta-EGFP

    Plasmid

    #136998

    Purpose

    Lentiviral EGFP lipid sensor for PI(4,5)P2

    Depositor

    Ken-Ichi Takemaru

    Insert

    PH-PLC-delta-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NanoLuc-PM-Venus

    Plasmid

    #164786

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    

    Use

    Tags

    NanoLuc and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mWasabi-C1

    Plasmid

    #54743

    Purpose

    Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mWasabi

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-mScarlet-EFS-Puro

    Plasmid

    #227259

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge