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V5-LOV-Turbo1-NIK3x_pTRE-G418

Plasmid

#202017

Purpose

expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-NIK3x

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE141

Plasmid

#118493

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-yibDp-Erv1p

Plasmid

#202483

Purpose

Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PIK3CA-WT (pFastBac)

Plasmid

#16644

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-PGAL1

Plasmid

#41605

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1360

Plasmid

#119278

Purpose

for cloning new sgRNAs

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-3HA

Plasmid

#39351

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA and kanMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-PGAL1-GST

Plasmid

#41611

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-TWIST2

Plasmid

#197935

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Use

Tags

3xFLAG tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSG020

Plasmid

#126503

Purpose

Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpGgi with 6xHis-tag

Use

Tags

C-terminal 6xHis

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

Plasmid

#205022

Purpose

mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Use

Tags

Streptag II

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

Plasmid

#164262

Purpose

Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1171

Plasmid

#119253

Purpose

rfp "test" strain

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1170

Plasmid

#119252

Purpose

rfp "test" strain

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge