We narrowed to 4,065 results for: p2a

1. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Expression

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKnockinDonor-human-p16INK4A-P2A-mCherry

   Plasmid

   #137026

   Purpose

   donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

   Depositor

   Chunliang Li

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

   Insert

   p16INK4A-knockin-homology arm-P2A-mCherry
   

   Use

   Tags

   P2A-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

   Plasmid

   #209298

   Purpose

   A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.

   Depositor

   Birgitt Schuele

   Article

   Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1

   Inserts

   dCas9
   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-inducible promoter (TRE3G)

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

   Plasmid

   #170229

   Purpose

   Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1 actin-3XNLS P2A mCherry

   Plasmid

   #58475

   Purpose

   Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-nbt-mTagBFP2-P2A-mScarlet

   Plasmid

   #196115

   Purpose

   zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-mTagBFP2-P2A-mScarlet
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKIBra-P2A-mCherry-Puro-sfGFP

   Plasmid

   #115686

   Purpose

   Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

   Depositor

   David Suter

   Article

   Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

   Insert

   P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIV-NAT-FLAG-CIP2A FL (sg2R)

   Plasmid

   #222624

   Purpose

   Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha promoter

   Available since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLVH-EF1a-GFP-P2A-SOX2

   Plasmid

   #130693

   Purpose

   Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

    Plasmid

    #112006

    Purpose

    For co-expression of GCaMP6s with mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. BSD-P2A-TagRFP-hSmad1-donor

    Plasmid

    #203662

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    smad1 (SMAD1 Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

    Plasmid

    #189807

    Purpose

    Retroviral delivery of Cas9

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid

    #223136

    Purpose

    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized PEmax-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

    Expression

    Mammalian

    Mutation

    M-MLV mutations from PE2 (Anzalone et al. Nature …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-mCherry-P2A-strepII-PPPCB

    Plasmid

    #67923

    Purpose

    Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2-SpG-P2A-GFP

    Plasmid

    #159980

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG-P2A-GFP
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK453 (BSD-P2A-dTAG-mCherry)

    Plasmid

    #214383

    Purpose

    dTAG-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92389

    Purpose

    expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-SOUL-P2A-tdTomato

    Plasmid

    #153019

    Purpose

    Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    P2A-tdTomato

    Expression

    Mammalian

    Mutation

    C128S/D156A/T159C

    Promoter

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

    Plasmid

    #232640

    Purpose

    Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

    Depositor

    Katherine Rogers

    Insert

    Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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