We narrowed to 173,905 results for: Gene
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Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 S383A
Plasmid#27160PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domainDepositorAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_hsNotch3 LNR
Plasmid#188338PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FAP site with scFv
Plasmid#188351PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV+ episome
Plasmid#169742PurposeCMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CAG/WPREΔU3
Plasmid#190727PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralExpressionMammalianPromoterCAGAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-HygTK-L2
Plasmid#141006PurposeMutisite Gateway mediated vector constructionDepositorInsertHygTK
ExpressionMammalianAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-TV
Plasmid#158409PurposeCRISPR-dpcoCas9 fused with TV activator for transcriptional activationDepositorInsertdpcoCas9-TV(6xTAL-2xVP64)
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
M-SPn-VP64
Plasmid#48674PurposeMammalian SP-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64Mutationnuclease-nullPromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
NDUFV3_pCSdest
Plasmid#53889DepositorAvailable SinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHygromycin (GB0211)
Plasmid#68204PurposeProvides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid partDepositorInsertHygromycin resistance gene coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMVSP6-NR2F2-SV40-PURO
Plasmid#138361PurposeLentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selectionDepositorInsertHuman NR2F2, with puromycin driven by SV40 for selection
ExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IQ013: pMVP/Ad-DEST
Plasmid#121843PurposepMVP destination vector, empty adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FnIII-Notch
Plasmid#188342PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE491
Plasmid#149292PurposeT-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2DepositorInsertp35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQ398X-EGFP
Plasmid#68849Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
TagsEGFPExpressionMammalianMutationQ398X, results in truncation of the protein into …PromoterCMVAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLC-241
Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only