We narrowed to 28,500 results for: REP

1. pMXs-hM₃O-IP

   Plasmid

   #46645

   Depositor

   Nobuaki Kikyo

   Article

   Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

   Insert

   hM₃O (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   IRES Puro and human MyoD (aa 1-62)

   Expression

   Mammalian

   Mutation

   contains a fragment of human MyoD (aa1-62) fused …

   Promoter

   Available since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

   Plasmid

   #126697

   Purpose

   donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-BAR-YR

   Plasmid

   #61766

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Bar gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-21-5p

   Plasmid

   #103345

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-21-5p target (MIR21 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-45b-HOXD13(+7A)-IDR-mCherry

   Plasmid

   #145281

   Purpose

   Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+7A)-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRLuc-CoV2-FLucStop

   Plasmid

   #177617

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   In-frame stop codon added between FLuc and FSE

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSB-hsa-miR-30d-5p

   Plasmid

   #103417

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-30d-5p target (MIR30D Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LSB-hsa-miR-132-3p

   Plasmid

   #103219

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-132-3p target (MIR132 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJOP-HTT-HR18Q

   Plasmid

   #87228

   Purpose

   HTT HR arms with 18 CAG repeats and piggyBac selection cassette

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a driving selection cassette

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVH-EF1a-GFP-P2A-OCT4

    Plasmid

    #130692

    Purpose

    Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LSB-hsa-miR-27a-3p

    Plasmid

    #103381

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-27a-3p target (MIR27A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-FUW-Hes1

    Plasmid

    #61534

    Purpose

    Expresses Hes1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBRY-nuclear mCherry-IRES-PURO

    Plasmid

    #52409

    Purpose

    Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    nuclear mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. LSB-hsa-miR-133b

    Plasmid

    #103223

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-133b target (MIR133B Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-TetO-Tead4

    Plasmid

    #128832

    Purpose

    dox-dependent expression of mouse Tead4

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Tead4 (Tead4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-GG-OCT4-1-5-PGK-Puro

    Plasmid

    #102893

    Purpose

    PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCyCEN_Lisp2mCherry_hsp70_GFP

    Plasmid

    #137169

    Purpose

    Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

    Depositor

    Clemens Kocken

    Article

    Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

    Inserts

    mCherry
    Nanoluc
    dihydrofolate reductase
    Green Fluorescent Protein
    

    Use

    Unspecified

    Tags

    T2A

    Expression

    Mutation

    Promoter

    P. cynomolgi hsp70 and P. cynomolgi lisp2

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

18. PB-TET-mKLF4

    Plasmid

    #20906

    Purpose

    piggyBac vector with tet inducible mKLF4

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    KLF4 (Klf4 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid

    #110626

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-4eCOL2A1

    Plasmid

    #97211

    Purpose

    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Vector is designed for cloning and generation of …

    Tags

    Expression

    Mutation

    Promoter

    COL2A1 regulatory elements

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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