20,361 results
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pSMALB-ATF4.5
Plasmid#155032PurposeFluorescent reporter for ATF4 translationDepositorAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαi1-LgB91 (GNAI1 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPR-HOT-Clover-BlastR
Plasmid#138569PurposeUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-BlastR
UseCRISPRAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
TagsHA and mycExpressionMammalianPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE3G-Bi-α-chain
Plasmid#133730PurposeExpresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsa-Chain_gePSI
ZsGreen1
ExpressionMammalianMutationOnly one part of the gePSI-system (aa1-141 + stop…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-RFP
Plasmid#127347PurposeExpresses RFP constitutively via the CAGGS promoter in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRed Fluorescent Protein
ExpressionMammalianPromoterCAGGS PromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W
Plasmid#112925PurposeLentiviral vector for SAM-CRISPRa gRNA expression with puroBFPDepositorInserthU6 promoter, gRNA-SAM expression cassette
UseCRISPR and LentiviralExpressionMammalianAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tsPurple chromoprotein
Plasmid#117848PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, tsPurple
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-SaCas9-U6-sgSlc17a6
Plasmid#124847PurposeMutagenesis of Slc17a6 with SauCas9DepositorInsertSlc17a6 gRNA (Slc17a6 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-RAD50
Plasmid#116784PurposeLentiviral expression of RAD50DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
EB3-mNeonGreen
Plasmid#98881PurposeIn vivo visualization of microtuble plus ends (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3
TagsmNeonGreenExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR GFP-CaaX
Plasmid#113020Purposefor lentivirus production of GFP-CaaXDepositorInsertGFP-CaaX
UseLentiviralExpressionMammalianAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti CMVie-IRES-BlastR
Plasmid#119863Purpose3rd generation lentiviral expression under a CMVie promoter with Blasticidin selectionDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterCMV immediate earlyAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1/CIP2A(1-905) WT V5 His
Plasmid#119287PurposeCIP2A WT V5 overexpressionDepositorAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron-ST
Plasmid#119034PurposeExpress soma-localized Voltron in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVoltron-ST
ExpressionMammalianPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-ExRai-AKAR
Plasmid#118405PurposecpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExRai-AKAR
ExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-hph
Plasmid#117232PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
