We narrowed to 17,814 results for: She

1. HsCLYBL(aa23-340) D320N-His

   Plasmid

   #111289

   Purpose

   bacterial expression

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

   Plasmid

   #141395

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

   Plasmid

   #213009

   Purpose

   A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-TetOne-Puro-p21

   Plasmid

   #171122

   Purpose

   Doxycycline inducible expression of p21

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   p21 (CDKN1A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

   Plasmid

   #131004

   Purpose

   bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   Kv2.1-HA

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PMmEos3.2

   Plasmid

   #203755

   Purpose

   Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Lyn n-term (LYN Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HsTPC2-EYFP

   Plasmid

   #135194

   Purpose

   Expresses tagged TPC2 in mammalian cells

   Depositor

   Antony Galione

   Article

   Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

   Insert

   TPC2 (TPCN2 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-SIN-BX SpyCatcher003-hCD52

   Plasmid

   #210565

   Purpose

   Expresses Spycatcher003 on the cell surface using human CD52 hinge

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to human CD52 hinge
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pF145 pAcGFP1 SOD1G93A

    Plasmid

    #26406

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G93A

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

    Plasmid

    #216386

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pF142 pAcGFP1 SOD1 A4V

    Plasmid

    #26403

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A4V

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pF150 pSOD1G93AAcGFP1

    Plasmid

    #26411

    Purpose

    contains G94A mutation in pSOD1

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G94A

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR221 UBR5 ∆UBR

    Plasmid

    #81063

    Purpose

    Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
    

    Use

    Cloning

    Mutation

    W1235L (bp g3704t)

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB-Tet-On-Arl13b-RhoA sensor

    Plasmid

    #189613

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Arl13b
    

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Promoter

    Tet3G

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plv-AcGFP-SOD1 (G93A)

    Plasmid

    #27142

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    AcGFP

    Mutation

    G93A

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Syn1-GRAB-HTR6-cilia

    Plasmid

    #189615

    Purpose

    Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6_GRAB
    

    Use

    AAV

    Promoter

    Syn1

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SHC003BSD-EXOC7

    Plasmid

    #133298

    Purpose

    Expresses EXOC7 in mammalian cells

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Insert

    Exocyst complex component 7 (EXOC7 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MmEos3.2

    Plasmid

    #203754

    Purpose

    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Src15 n-term (SRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Chr1q_Cassette-Integration_gRNA

    Plasmid

    #195126

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

    Plasmid

    #216387

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-TetOne-Puro-PFDN2

    Plasmid

    #202664

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    prefoldin subunit 2 (PFDN2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pF144 pAcGFP1 SOD1G85R

    Plasmid

    #26405

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G85R

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

25. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

26. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mEos3.2FP

    Plasmid

    #203757

    Purpose

    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    NRas c-term (NRAS Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FusionRed-Fibrillarin-7

    Plasmid

    #56118

    Purpose

    Localization: Nucleoli, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. OR2M4_Deletion_gRNA

    Plasmid

    #195194

    Purpose

    dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR2M4 dual gRNA (OR2M4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pF143 pAcGFP1 SOD1G37R

    Plasmid

    #26404

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G37R

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

34. FusionRed-Caveolin-C-10

    Plasmid

    #56103

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

35. FusionRed-CD36-C-10

    Plasmid

    #56104

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CD36 (CD36 Human)
    

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

36. pAAV-TRE-HTR6-SNAPf-TRIP

    Plasmid

    #189617

    Purpose

    Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6-TRIP_alpha
    

    Use

    AAV

    Tags

    alphaTag in the N-term of HTR6, SNAP-tag in the C…

    Promoter

    TRE

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

38. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pGL3-DICER-Prom Ebox double mut

    Plasmid

    #25854

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter Ebox double mut (DICER1 Human, gene ID 23405)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge