We narrowed to 164,714 results for: DES

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pDESTFLAGHApuro3-Ago2Q545AK570A

Plasmid

#14105

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Gln545 to Ala and Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDest-311 (attR4-attR3)

Plasmid

#161896

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-663 (attR4-attR2)

Plasmid

#161884

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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LC001: pMVP/PB/Hygro-DEST

Plasmid

#121876

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-KP1

Plasmid

#206504

Purpose

AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

KP1 capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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BCL2L1_pLX307

Plasmid

#98323

Purpose

Lentiviral expression of BCL2L1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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BMP4_pLX307

Plasmid

#98321

Purpose

Lentiviral expression of BMP4

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BMP4 (BMP4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-AIM2

Plasmid

#73958

Purpose

Express c-Myc-tagged human AIM2 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

Insert

absent in melanoma 2 (AIM2) (AIM2 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mStrawberry

Plasmid

#161734

Purpose

Doxycycline-inducible expression of mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

mStrawberry

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:icre-p2a-tomato

Plasmid

#135217

Purpose

Cre coexpressed with tdTomato in macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter with iCre and Tomato

Use

Cre/Lox; Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAIP

Plasmid

#74171

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-Clover-BlastR

Plasmid

#138569

Purpose

Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

Clover-BlastR

Use

CRISPR

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _PDZ

Plasmid

#59147

Purpose

YAP1 mutants that harbored a deletion of the 5 amino acids

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ binding domain (last 5aa) deleted

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FAS_pLX307

Plasmid

#98334

Purpose

Lentiviral expression of FAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FAS (FAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-6

Plasmid

#72346

Purpose

Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) IGF1R

Plasmid

#107499

Purpose

Expresses IGF1R, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

IGF-1R (IGF1R Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only