We narrowed to 45,011 results for: cha;
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Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502_sgCD4
Plasmid#158704PurposeCD4 CRISPRa controlDepositorInsertCD4 (CD4 Human)
UseLentiviralAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
ExpressionWormPromoterlim-4Available SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT FUCCI
Plasmid#136935Purposedonor plasmid for cardiac-specific FUCCI expression in human cellsDepositorInsertTroponin T promoter
UseSynthetic BiologyExpressionMammalianPromotercTnTAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
TagsGSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
dRT-pMDLg/pRRE
Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
Mutationchanged Asp at position 249 + 250 to ValAvailable SinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1-mScarlet
Plasmid#211330PurposeMammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2
Plasmid#99126PurposeAn AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterhSyn1Available SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-MXT GIRK2
Plasmid#20675DepositorInsertMus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
UseXenopus expressionTags3' UTR Xenopus B- globin and 5' UTR Xen…MutationA369VAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR76
Plasmid#202776Purposeepisomal fluorescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
UPB-colon (UPB4)
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVExpressionMammalianPromoterCAGAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only