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Showing: 9441 - 9460 of 31286 results

  1. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  2. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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  3. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114075-plasmid-map-sequence-id-220517

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  5. PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla

    Plasmid
    #199551
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1β-hHNF4α-hHNF6 (ONECUT1, HNF4A, HNF1B Human)
    Use
    Piggybac transposon vector
    Expression
    Mammalian
    Promoter
    TRE+CMVmin promoter
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199551-plasmid-map-sequence-id-405450

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  6. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid
    #197500
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197500-plasmid-map-sequence-id-386637

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  7. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  8. pMini-CMV-NLS-R-IscB-VR4-NLS (D60A, H243E, H269N, R270Q)_T2A_mCherry_U6-ωRNA

    Plasmid
    #246431
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. ssRNase enhancing mutations (H243E, H269N, R270Q) included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB-VR4 (H243E, H269N, R270Q) with RuvC dead mutations (D60A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    246431-plasmid-map-sequence-id-496263

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  11. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid
    #246436
    Purpose
    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    Tags
    GFP N-terminal sequence (M1 to Q95), Hemi Intron …
    Expression
    Mammalian
    Mutation
    GFP N-terminal sequence M1 to Glutamine 95 append…
    Promoter
    CMV and U1a
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246436-plasmid-map-sequence-id-490914

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  12. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid
    #200673
    Purpose
    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200673-plasmid-map-sequence-id-391864

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  14. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

    Plasmid
    #201560
    Purpose
    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.
    Depositor
    Randy Poon
    Article
    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754
    Insert
    DBEcR and EGFP
    Use
    Unspecified; Sleeping beauty transposon
    Tags
    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD
    Expression
    Mammalian
    Promoter
    EF1a and pIND(SP1)
    Available Since
    July 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    201560-plasmid-map-sequence-id-400950

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  15. pDSP1

    Plasmid
    #199384
    Purpose
    Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4
    Depositor
    Hang Lu
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    Cloning vector backbone
    Available Since
    April 20, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199384-plasmid-map-sequence-id-390594

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  16. pMal-Halo

    Plasmid
    #209349
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209349-plasmid-map-sequence-id-419545

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  20. Topaz-pBAD

    Plasmid
    #54623
    Purpose
    Localization: Protein Expression Vector , Excitation: 514, Emission: 527
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and Topaz
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9441 - 9460 of 31286 results