We narrowed to 15,384 results for: pET

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pAAV_-CMV-Pcdh15_mini_V4-CD2

Plasmid

#199184

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V4-CD2

Use

AAV

Mutation

EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.3#3/Cre

Plasmid

#173662

Purpose

Expresses a NT.3-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTAL_RFP

Plasmid

#43859

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Type

Empty backbone

Expression

Mammalian

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-IFITM3g1 (BB15)

Plasmid

#139460

Purpose

Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTAL_GFP

Plasmid

#43858

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Type

Empty backbone

Expression

Mammalian

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW1,2,4

Plasmid

#171137

Purpose

expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L (NEDD4L Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine, tryptophan 453…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_Smc4-mAID-HaloTAG

Plasmid

#217658

Purpose

Repair template to endogenously tag SMC4 with mAID-Halo Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

SMC4-mAID-HaloTag (SMC4 Human)

Expression

Bacterial and Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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hEBI3 pEF6-V5 (MJS_002)

Plasmid

#72490

Purpose

Expression of human EBI3 in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

EBI3 (EBI3 Human)

Tags

His/V5

Expression

Mammalian

Promoter

EF-1 alpha

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#40777

Purpose

Expression of rat Erk2 in mammalian cells

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

Erk2 (Mapk1 Rattus norvegicus)

Tags

eGFP

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pRC/CMV-JAK1P1084A-VSV

Plasmid

#139358

Purpose

Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Tags

VSV

Expression

Mammalian

Mutation

P1084A

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

Plasmid

#114102

Purpose

fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

Insert

GLucM23-iChloC-EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVenus-CyclinB1(R42A) (1317)

Plasmid

#39873

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

CyclinB1(R42A) (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

R42A = destruction box mutations = non-destructib…

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRF+275Dux4

Plasmid

#21293

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX 4 (DUX4 DUX 4, Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pmiRGLO-ADGRL1-201

Plasmid

#117336

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR ADGRL1-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0004

Plasmid

#42244

Purpose

gRNA targeted to zebrafish gene fh [site #2]

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-fh site #2 (fh Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only