165,688 results

1. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKC delta DN

   Plasmid

   #16389

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta isoform 2 (Prkcd Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Dominant negative (kinase inactive): K376R

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-IRES-Puro GFP-3xFLAG

   Plasmid

   #201636

   Purpose

   Over-expression of 3xFLAG-tagged GFP

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5 HA-TBRII (delta Cyt)

   Plasmid

   #14051

   Depositor

   Joan Massague

   Article

   Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

   Insert

   TGF beta rec II (delta cyt) (TGFBR2 Human)
   

   Tags

   HA (PYDVPDYA)

   Expression

   Mammalian

   Mutation

   Dominant negative mutant. A stop codon was introd…

   Available Since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Cys-3HB-SPYcatcher
   

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-yomRuby2-Kan

   Plasmid

   #44953

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomRuby2
   

   Expression

   Yeast

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mScarlet-EFS-Blast

   Plasmid

   #227260

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGG-NFIC2

    Plasmid

    #112701

    Purpose

    Expression of NFIC2 protein and GFP

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I C2 (Nfic Mouse)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p177bp-LacOx50-Hyg

    Plasmid

    #210213

    Purpose

    Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LARS_pNIC-Bio3

    Plasmid

    #153052

    Purpose

    Expression construct for producing Avi tagged Leucyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    LARS (LARS1 Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Not full length protein

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-RIAM

    Plasmid

    #32803

    Depositor

    Vicki Boussiotis

    Insert

    RIAM (APBB1IP Human)
    

    Tags

    His and XPress

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS-DSCP

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid

    #155045

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Expression

    Mammalian

    Promoter

    CMV, SP6 and U6

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXB1-nbMmKappa-DddA11_NT

    Plasmid

    #232442

    Purpose

    Anti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

    Depositor

    Ivan Raimondi

    Article

    Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

    Insert

    nbMmKappa-DddA11_NT
    

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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