165,688 results

1. pcDNA3-Xl-VSP1

   Plasmid

   #51880

   Purpose

   expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. GRB2-V5

   Plasmid

   #139631

   Purpose

   V5-His-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Tags

   3xV5-6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mGreenLantern

   Plasmid

   #197928

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   mGreenLantern
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

   Plasmid

   #73796

   Purpose

   pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

   Depositor

   Ronald Davis

   Article

   Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

   Inserts

   dCas9-Mxi1
   Tet Repressor
   Structural gRNA for S pyogenes
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1, pRPR1(TetO), and pTef1

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ornithine transcarbamylase (OTC)

   Plasmid

   #71877

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC (Otc Rat)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIVTRup

   Plasmid

   #101362

   Purpose

   Highly efficient in vitro transcription. Empty backbone.

   Depositor

   Ángel Raya

   Insert

   5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
   

   Use

   For cloning a gene for in vitro transcription pur…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_CBR1_WT_V5

   Plasmid

   #82945

   Purpose

   Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CBR1 (CBR1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PPRE-H2B-eGFP

   Plasmid

   #84393

   Purpose

   Fluorescent reporter for PPARgamma activity

   Depositor

   Thierry Fournier, Severine Degrelle

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   PPRE

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Promoter

    cx55.5

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUX19

    Plasmid

    #36328

    Depositor

    Gary Roberts

    Type

    Empty backbone

    Use

    Suicide vector for r. rubrum

    Expression

    Bacterial

    Promoter

    reduce strength lacZ

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

13. 1572 pSG5L Flag HA GGA2

    Plasmid

    #11184

    Depositor

    William Sellers

    Insert

    GGA2 (GGA2 Human)
    

    Tags

    HA and flag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    human OCT4

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUW-tetO-Batf3

    Plasmid

    #139837

    Purpose

    doxycycline-inducible expression of mouse Batf3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Batf3 (Batf3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Expression

    Bacterial

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDDGFP_LEU2D

    Plasmid

    #58352

    Purpose

    Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

    Depositor

    Simon Newstead

    Article

    Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GFP-His

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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