4,178 results

1. pLVX-puro-cOVA-IRES-BFP

   Plasmid

   #135074

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

   Depositor

   Maria Castro

   Insert

   cOVA-IRES-BFP (OVAL Chicken)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1(+)-CMV-bArrestin2-TEV

   Plasmid

   #107245

   Purpose

   Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ARRB2
   

   Tags

   TEV(NIA), tobacco etch virus NIA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-Streptavidin-Dead

   Plasmid

   #20859

   Purpose

   Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Dead
   

   Expression

   Bacterial

   Mutation

   Triple mutant of wt Streptavidin N23A,S27D,S45A

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 F-tractin-EGFP

   Plasmid

   #58473

   Purpose

   Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   F-tractin (Itpka Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPgRNA

   Plasmid

   #47108

   Purpose

   Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   SPgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIRV-NFAT-eGFP

   Plasmid

   #118031

   Purpose

   Fluorescent reporter for NFAT activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eGFP
   

   Use

   Retroviral

   Promoter

   minimal promoter with NFAT binding site

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. MaLionG

   Plasmid

   #113906

   Purpose

   Green ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionG
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

    Plasmid

    #61762

    Purpose

    Dual Color betaGlobin reporter to study splicing kinetics in vivo

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    b-Globin
    

    Tags

    24 X MS2 hairpin cassette in 3'UTR of b-Glob…

    Expression

    Mammalian

    Promoter

    Dox inducible Tet-ON promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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