4,179 results

1. CD63-pEGFP C2

   Plasmid

   #62964

   Purpose

   full length human CD63 cloned into pEGFP C2

   Depositor

   Paul Luzio

   Insert

   CD63 molecule (CD63 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CD63 has 1 silent mutation compared to the coding…

   Promoter

   CMV

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_502

   Plasmid

   #96923

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamp1-mScarlet-I

   Plasmid

   #98827

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lamp1
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha and EF1-alpha

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XFlag-pA-Tn5-Fl

   Plasmid

   #124601

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Steven Henikoff

   Article

   Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

   Insert

   Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
   

   Tags

   Protein A and Tn5 transposase fusion protein with…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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