We narrowed to 22,307 results for: CRISPR

1. pMZ285

   Plasmid

   #52226

   Purpose

   Expression of sgRNA precursor targeted against ade6M210

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. FISSEQ-hgRNA-Design1

   Plasmid

   #100571

   Purpose

   FISSEQ-hgRNA-Design1 in a lentiviral backbone

   Depositor

   George Church

   Article

   Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

   Insert

   FISSEQ-hgRNA-Design1
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-U6-BRD4-HaloTag-Guide-hspCas9

   Plasmid

   #228576

   Purpose

   Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   BRD4 gRNA (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC055

   Plasmid

   #225977

   Purpose

   dRxCas13d expressed from pTet promoter

   Depositor

   Jesse Zalatan

   Article

   Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

   Insert

   dRxCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pTet

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNT-15

   Plasmid

   #154932

   Purpose

   15 designed non-target sites

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   15 designed non-target sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNT-12

   Plasmid

   #154933

   Purpose

   12 designed non-target sites

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   12 designed non-target sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNT-3

   Plasmid

   #154934

   Purpose

   3 designed non-target sites

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   3 designed non-target sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNT-1

   Plasmid

   #154935

   Purpose

   1 designed non-target site

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   1 designed non-target sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMZ286

   Plasmid

   #52227

   Purpose

   Expression of sgRNA precursor targeted against ade6L469

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPN10-gDM1d

    Plasmid

    #114387

    Purpose

    pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

    Depositor

    Vincent Dion

    Article

    Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

    Insert

    gDM1d
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGW_RUBY_EggCas9

    Plasmid

    #225990

    Purpose

    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_ZNF3

    Plasmid

    #72362

    Purpose

    Donor vector for 3' FLAG tag of human ZNF3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF3 Homology Arms (ZNF3 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid

    #220961

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR6K-GFP-CASTIB

    Plasmid

    #199655

    Purpose

    R6K plasmid with a I-B CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-B systems
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR6K-GFP-CASTV

    Plasmid

    #199657

    Purpose

    R6K plasmid encoding a type V CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST V systems
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pXZ13lacZalphaKanR

    Plasmid

    #160230

    Purpose

    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRGEB32

    Plasmid

    #63142

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSGKP-km

    Plasmid

    #117233

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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