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pPTK001-2-pAOX1

Plasmid

#84960

Purpose

Type 2, pAOX1, Alcohol oxidase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86206

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBXNAC3GH

Plasmid

#47073

Purpose

FX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, GFP…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBbE2c-RFP

Plasmid

#35323

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0257

Plasmid

#162308

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame2 (TTC-G)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0281

Plasmid

#162302

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, [S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Tags

mito::GCaMP3

Expression

Worm

Promoter

Pcol-19

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHnCB

Plasmid

#52064

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDXNSM3

Plasmid

#49028

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_19

Plasmid

#64045

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS5c-RFP

Plasmid

#35284

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

March 14, 2012

Availability

Academic Institutions and Nonprofits only

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pGL0_21 [UARS1 ORI]

Plasmid

#198934

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

UARS1 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EcAvs2

Plasmid

#188789

Purpose

Avs2 from Escherichia coli NCTC9087

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2367

Plasmid

#160076

Purpose

Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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