We narrowed to 19,203 results for: IRE

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla 3A

Plasmid

#84872

Purpose

Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Tags

His

Expression

Bacterial

Mutation

3 C-terminal leucines have been replaced by alani…

Promoter

T7

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

Plasmid

#164235

Purpose

lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 1-234

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-GLIS1 (FLJ33315SAAN)

Plasmid

#33274

Purpose

Retroviral expression of canonical human GLIS1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (GLIS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8

Plasmid

#135512

Purpose

Mammalian expression of myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKG139

Plasmid

#69755

Purpose

Retroviral expression of CENP-C

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-C (CENPC Human)

Use

Retroviral

Tags

mCherry-TEV-S

Expression

Mammalian

Mutation

L341F, N570D relative to NP_001803.2 (numbering b…

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Promoter

sp6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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AMPK-β2-MYC

Plasmid

#40606

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35514

Purpose

Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArch3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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ETV1_pet28a

Plasmid

#131644

Purpose

Bacterial expression of ETS gene ETV1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV1 (ETV1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinE442Q

Plasmid

#134464

Purpose

M1 SpastinE442Q expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Expression

Mammalian

Mutation

Glu to Gln at amino acid 442

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.hLHX6

Plasmid

#161828

Purpose

Expresses human LHX6(LIM Homeobox 6) in mammalian cells

Depositor

Daniella Ottosson

Article

Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

Insert

human LHX6 (LHX6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AA 17-23 deletion

Promoter

PGK

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

Plasmid

#188991

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 CS

Plasmid

#8319

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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Nrxn3α-AS4+

Plasmid

#184333

Purpose

Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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