We narrowed to 17,265 results for: tes
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Plasmid#215108PurposeExpresses p21-HA(dPCNA) in mammalian cells.DepositorInsertCDKN1A (CDKN1A Human)
TagsHAExpressionMammalianMutationp21(dPCNA) denotes mutations M147A, D149A, F150A.PromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13 5xBoxB
Plasmid#207128PurposeTethered luciferase reporter for mouse Il13 BoxBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInterleukin 13 (Il13) 3'UTR (Il13 Mouse)
UseLuciferaseTags5xBoxBExpressionMammalianAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PHOT1
Plasmid#209187PurposepENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tagsDepositorPublicationAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]
Plasmid#196885PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-NEDD1-gTBD
Plasmid#196858PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmOrange2-N-gTBD (NEDD1 Human)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV494
Plasmid#177701PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), SNR52p (Candida s…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTCRA (Tcra Mouse)
ExpressionMammalianPromoterCMV and T7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_deltaOB
Plasmid#166408Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPOT1 ΔOB (POT1 Human)
TagsmycExpressionMammalianMutationΔOBAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)
Plasmid#160619PurposeModule for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.DepositorInsertERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-decto
Plasmid#86238PurposeLentivirus for expression of human Plexin-B2 with deletion of ectodomainDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationPLXNB2 with deletion of extracellular domainPromoterhPGKAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-mRBD
Plasmid#86240PurposeLentivirus for expression of human Plexin-B2 with mutant RBD domainDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationPLXNB2 with point mutations of RBD domainPromoterhPGKAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-D2-B/c
Plasmid#137883PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseGateway-compatible entry cloneMutationA. thaliana TAS1c precursor sequence including a …Available sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-MC38-TCRA-33-MC_hammer
Plasmid#138989PurposeIVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTCRA (Tcra Mouse)
ExpressionMammalianPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB63 - pL0_fLUC-I (CDS1)
Plasmid#123180PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plantsDepositorInsertLuciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
UseLuciferase; Part for plant expressionExpressionPlantMutationNo BpiI and BsaI sitesAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-HF2-BE2
Plasmid#120396PurposeExpresses HF2-BE2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHF2-BE2
ExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-mFzd4
Plasmid#102866PurposeExpresses mouse Fzd4 in mammalian cellsDepositorInsertFrizzled 4 (Fzd4 Mouse)
UseLentiviralExpressionMammalianMutationT to C substitution at nucleotide 1149 of Fzd4 OR…PromoterEF1AAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
Plasmid#69050Purposelentiviral expression of IKZF3 with Q147H mutation and FLAG tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationQ147HPromoterCAGAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 N-t
Plasmid#89980PurposeExpresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertataxin-3 (ATXN3 Human)
TagsGFPExpressionMammalianMutationconstruct contains aa 1-182; deleted aa 183-361Available sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-MKOS
Plasmid#80484PurposepiggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianPromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL ∆CC
Plasmid#80268Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTIL (STIL Human)
TagsMyc-GFPExpressionMammalianMutationmissing conserved coiled-coil (amino acids 721-74…PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only