We narrowed to 3,430 results for: Agt

1. pUC19-U6-AasgRNA3.8.3

   Plasmid

   #121959

   Purpose

   Mammalian expression, Transcription regulation, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA, MS2 hairpin inserted
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVenus-PICK1-shRNA

   Plasmid

   #72575

   Purpose

   Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

   Depositor

   Victor Anggono, Richard Huganir

   Article

   Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

   Insert

   PICK1 (Pick1, Pick1 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

   Plasmid

   #114731

   Purpose

   sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   sgRNA M9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNA[bTub].1026C

   Plasmid

   #112691

   Purpose

   express gRNA targeting bTub under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   dU6-3

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmEos2-Linker_PCNA-MutC

   Plasmid

   #98272

   Purpose

   for low level expression of mEos2-PCNA in mammalian cells

   Depositor

   Mike Heilemann

   Article

   Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRRNA-G0-R20

   Plasmid

   #101818

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRRNA-G20-R0

   Plasmid

   #101807

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   Sven van Teeffelen, David Bikard

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pATI (pKD-G7)

   Plasmid

   #62686

   Purpose

   Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   amiR-eGFP123/amiR-eGFP419
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. hIRF-1 gRNA #409 (Sa)

   Plasmid

   #98134

   Purpose

   Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Insert

   gRNA_hIRF1 promoter #409
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hIRF-1 gRNA #351 (Sa)

    Plasmid

    #105283

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #351
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pATM (pKD-G11)

    Plasmid

    #62690

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP123/amiR-eGFP419/tdTomato
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-H1-sgRNA-hTZAP

    Plasmid

    #87186

    Purpose

    guideRNA targeting exon1 of human TZAP

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pADH118-17

    Plasmid

    #91727

    Purpose

    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pADH143-2

    Plasmid

    #90990

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADH100-2

    Plasmid

    #90981

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-sgRNA_Dgcr8_4

    Plasmid

    #73528

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pSuper-TatSF1 AB

    Plasmid

    #31119

    Depositor

    Mariano Garcia-Blanco

    Article

    Miller et al PLoS One. 2009 . 4(5):e5710.

    Insert

    TatSF1 shRNA (HTATSF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

18. esgRNA_NbPDS

    Plasmid

    #231147

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbPDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCambia3301-AtU6-MAR1

    Plasmid

    #210757

    Purpose

    Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

    Depositor

    Daisuke Miki

    Article

    Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

    Insert

    AtU6-26 pro::MAR1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL3346 PB-nicking guide-blast

    Plasmid

    #226665

    Purpose

    PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    protective nicking sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. gRNA_SRR134.3_miRFP670

    Plasmid

    #163754

    Purpose

    gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR134.3
    

    Use

    CRISPR

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pITESceIcmd

    Plasmid

    #218288

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253, -1)-tSynth3

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

    Plasmid

    #218635

    Purpose

    Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    N1008R, R1009E, E1010R, R1013E

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLentiCRISPR-v2 hygro sgUCK1

    Plasmid

    #211524

    Purpose

    Deletes UCK1

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgUCK1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-RedO-shNC

    Plasmid

    #206356

    Purpose

    AAV plasmid expressing non-targeting control shRNA in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

    Insert

    Non-targeting control shRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. tet pLKO.1-shNUP37 v1 puro

    Plasmid

    #192343

    Purpose

    Lentiviral expression vector for an inducible shNUP37 v1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shNUP37 v1 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tet pLKO.1-shNUP37 v2 puro

    Plasmid

    #192344

    Purpose

    Lentiviral expression vector for an inducible shNUP37 v2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shNUP37 v2 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pORE303N_CEN1

    Plasmid

    #194439

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA targeting CEN1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MtU6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXPR_003 sgSNRPA guide 2

    Plasmid

    #193599

    Purpose

    SNRPA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgSNRPA guide 2 (SNRPA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge