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Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLRD11
Plasmid#208263PurposeProduction of sclareol in E. coli MEV20DepositorInsertstHMGR
abCAS
ssSS
UseTagsExpressionBacterialMutationabCAS(D537A)PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD14
Plasmid#208266PurposeProduction of syn-pimara-7,15-diene in E. coli MEV20DepositorInsertstHMGR
osCPS4
osKSL4
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD17
Plasmid#208269PurposeProduction of syn-stemod-13(17)-ene in E. coli MEV20DepositorInsertstHMGR
osCPS4
osKSL11
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-DDC
Plasmid#71723Purposedopa decarboxylase from pigDepositorInsertdopa decarboxylase
UseTagsExpressionMutationPromoterpTrcAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPH6
Plasmid#40315DepositorInsertPeptide:N-glycosidase F
UseTags6xHis and OmpA3ExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ231196Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MazF-WT
Plasmid#155202PurposeTo express recombinant MazF protein.DepositorInsertMazF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADS
Plasmid#19040DepositorInsertcodon-optimized synthetic amorphadiene synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMYO
Plasmid#34626DepositorInsertMyoglobin
UseTags6xHISExpressionBacterialMutationcodon-optimizedPromoterlppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
SJZ7-KAN
Plasmid#228260PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to G418DepositorInsertkan
UseTagsExpressionYeastMutationPromoterGPM1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaSeph
Plasmid#67507PurposeExpress the tRNAMet scaffold with the sephadex aptamer tagDepositorInsertmodified tRNAMet with sephadex aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSKrnaSeph
Plasmid#67506PurposeExpress the tRNALys scaffold with the sephadex aptamer tagDepositorInsertmodified tRNALys with sephadex aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIA030
Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsPAmCherryExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AC
Plasmid#108686PurposePlasmid for assembly of donor DNA templates consisting of two expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPB845
Plasmid#128296Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#128280Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF018 p3E-2A-mCerulean-CAAX
Plasmid#195975PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCerulean-CAAX (membrane)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC28
Plasmid#128284Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, sopC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKB230
Plasmid#170017PurposeEncodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Protegrin-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB226
Plasmid#170016PurposeEncodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Defensin HNP-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLRD04
Plasmid#208258PurposeProduction of ent-kaurene in E. coli MEV20DepositorInsertstHMGR
ptmT2
blr2150
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL630
Plasmid#167836PurposeEncodes OptoLAC3 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
Plasmid#30505DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2011AvailabilityAcademic Institutions and Nonprofits only