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We narrowed to 5,865 results for: pcDNA 3.1

Showing: 1441 - 1470 of 5865 results

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pcDNA3.1/Puro-CAG-JEDI-2P-Kv

Plasmid

#179462

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/myc-his Ndel1

Plasmid

#12572

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Puro-CAG-VSFP-CR

Plasmid

#40257

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

VSFP-CR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD9-(TEV)-tTA

Plasmid

#162596

Purpose

human CD9-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD9 (CD9 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (WT)

Plasmid

#73963

Purpose

STAG2 cDNA (CCDS43990) wild type

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

wild type

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1

Plasmid

#172648

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ GEMIN6 myc HIS

Plasmid

#71659

Purpose

Mammalian expression of GEMIN6

Depositor

Gideon Dreyfuss

Insert

GEMIN6 (GEMIN6 Human)

Use

Tags

HIS and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Myc-His RTN3A1

Plasmid

#31087

Depositor

Jimtong Horng

Article

Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

Insert

reticulon 3 (RTN3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

STIM1-Venus173-C (STIM1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Strep-Lrrk2-Myc

Plasmid

#161582

Purpose

expression of full-length mouse Lrrk2 with C-terminal Myc tag

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tau Authentic Alu 10-12V337M

Plasmid

#194169

Purpose

Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 V337M

Use

Tags

3X flag

Expression

Mammalian

Mutation

Changed Valine 337 to Methinonine

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Blue opsin mcherry ---- pcDNA3.1

Plasmid

#62932

Purpose

Blue opsin tagged with mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

blue opsin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-IDO2-p2a-eGFP

Plasmid

#187027

Purpose

Expresses IDO2 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Article

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 2 (IDO2 Human)

Use

Tags

P2A-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A CARD8 CARD-FLAG WT

Plasmid

#164020

Purpose

Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS3/myc-His

Plasmid

#127487

Purpose

Expresses myc-tagged NDUFS3 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS3 (NDUFS3 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Mito-AcGFP-MTn

Plasmid

#154350

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

8xHis and FLAG

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Rab8-T22N(Flag) in pcDNA3.1neo

Plasmid

#46784

Purpose

expression of T22N Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A T22N (RAB8A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T22N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only