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Showing: 1921 - 1960 of 40461 results

  6. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51283-plasmid-map-sequence-id-78362

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  10. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    U6 and CBh
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-shCTRL

    Plasmid
    #181875
    Purpose
    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    control shRNA with no known targets in the mouse genome
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181875-plasmid-map-sequence-id-355955

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  14. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    enhancer from 7SL gene
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

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  20. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Article
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Insert
    DD-dCas9VP192-T2A-EGFP
    Use
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69534-plasmid-map-sequence-id-122831

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  23. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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  24. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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  27. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  28. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

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  30. pBEG R2-iBlast-L3 #BGV204

    Plasmid
    #48982
    Purpose
    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    blasticidin-S deaminase
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pBEG R2-iHygro-L3 #BGV206

    Plasmid
    #48985
    Purpose
    Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    hygromycin phosphotransferase
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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  35. pEIAV-DIO-TLoop-CHR2YFP

    Plasmid
    #66798
    Purpose
    expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector
    Depositor
    Edward Callaway
    Article
    Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.
    Insert
    Floxed Chr2-YFP
    Use
    Lentiviral
    Tags
    citrine
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CMVTetO7 Promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    66798-plasmid-map-sequence-id-114340

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Showing: 1921 - 1960 of 40461 results