We narrowed to 41,926 results for: tro
-
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-E2_Crimson
Plasmid#172377PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertE2_Crimson
UseNeurotropic virusAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
KG#901
Plasmid#110936PurposeExpresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
aav-SYN-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113760PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterSynapsinAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-NLS
Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
ExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-FlpO
Plasmid#172378PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + FlpO
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP
Plasmid#11772DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R4) #BGV057
Plasmid#48957PurposeLentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)DepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-FlpO
Plasmid#172375PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + FlpO
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorPromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiAvailable SinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…Available SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-ChIEF
Plasmid#172370PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + ChIEF
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
LEP-shREN
Plasmid#105580Purposeretrovirally express control shRNA with puro resistance and GFP markerDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralExpressionMammalianPromoterMSCV-LTRAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hyper7 in pT3TS-Dest
Plasmid#194296PurposeIn vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRLDepositorInsertHyper7
UseIn vitro transcription of mrnaAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
RCAS-HA-b-actin
Plasmid#125834PurposeRetroviral expression of HA-tagged human beta-actinDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAP2-CD4
Plasmid#43916DepositorAvailable SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK267
Plasmid#129272Purpose"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed proteinDepositorInsertNLS-VP16-ZF 43-8-NES
Tags6xHAExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EYFP
Plasmid#172383PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEYFP
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-mCitrine
Plasmid#172384PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertmCitrine
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EGFP-ChIEF
Plasmid#172371PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEGFP + ChIEF
UseNeurotropic virusAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-FlpO
Plasmid#172379PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + FlpO
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-U6
Plasmid#173157PurposeSingle guide RNA cassette under U6 promoterDepositorInsertsgRNA cassette under U6 promoter
ExpressionPlantPromoterAtU6-26Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA}on-WPRE
Plasmid#111386PurposeAAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activationDepositorInsertChETA
UseAAVTagsHAExpressionMammalianMutationhumanized ChR2, E123T/H134RPromoterhSynapsinAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-SypRFP
Plasmid#172381PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertSynaptophysin1-RFP fusion protein
UseNeurotropic virusAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLS.416
Plasmid#187207PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorPromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8f
Plasmid#172387PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8f
UseNeurotropic virusAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-tdTomato
Plasmid#172382PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + tdTomato
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
Plasmid#48157PurposeTransgenically expresses firefly luciferase protein in T. cruziDepositorInsertluciferase
UseLuciferase; T. cruzi expressionPromoterT7Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEG R2-iCreT2ALuc-L3 #BGV208
Plasmid#48992PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertscre recombinase
Luciferase
UseLuciferase; Gateway cloning vectorTagsIRES (weak)PromoterUnknownAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorPromoterSV40Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-WT Split GFP
Plasmid#69891PurposeExpresses a GFP circular RNA in DrosophilaDepositorAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1 Insulator ObLiGaRe/EPB71
Plasmid#90018PurposeDonor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locusDepositorInsertMCS flanked by insulator and inverted ZFN binding sites
ExpressionBacterialAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM1162
Plasmid#112535PurposepZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
ExpressionBacterialPromoterJ23107 and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only