We narrowed to 4,562 results for: TIL
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Plasmid#172378PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + FlpO
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G2
Plasmid#40178DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b R1695M
Plasmid#134958PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.DepositorInsertMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
TagsmcherryExpressionMammalianPromoterCMV IEAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDLX-TVA-2A-N2cG
Plasmid#172364PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAVExpressionMammalianPromotermDLXAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_R
Plasmid#198277PurposeFor loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_R
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_mcherry
Plasmid#167886PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-ExSYTE
Plasmid#216263PurposeCre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoterDepositorInsertExSYTE P2A EGFP - Rat DTE
UseAAVAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS1051-Tier1-PhCMV-4xCox8
Plasmid#169490PurposeTier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)DepositorInsertPCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
ExpressionMammalianAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3HA
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G1
Plasmid#40177DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIC292
Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-FlpO
Plasmid#172375PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + FlpO
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…ExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
TagsEGFPExpressionMammalianAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pKeratin-miRFP670nano
Plasmid#127437PurposeKeratin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertKeratin-miRFP670nano
TagsmiRFP670nanoExpressionMammalianMutationKeratin V282L - please see depositor comments bel…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1-miR-30 L1-R5
Plasmid#62181PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MyosinIIA-C-18
Plasmid#54190PurposeLocalization: Cytoskeleton, Excitation: 487, Emission: 509DepositorInsertMyosinIIA
TagsmEmeraldExpressionMammalianPromoterCMVAvailable SinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5
Plasmid#60532PurposeDonor vector for human Pak5DepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateMutationThe 3’-end of the chloramphenicol resistance gene…Available SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2
Plasmid#169531PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).DepositorInsertPCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-ChIEF
Plasmid#172370PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + ChIEF
UseNeurotropic virusAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4
Plasmid#99018PurposeLocalization and visualization of trafficking protein Rab4DepositorAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57mini ZM249M P90A _env eGFP
Plasmid#132939PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequenceDepositorInsertHIV-1ZM249M provirus (P90A) with env deletion, GFP
UseLentiviralMutationP90AAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianPromoterhSynapsinAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only