We narrowed to 10,116 results for: gnas

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR SFFVp PHAkt Cerulean

Plasmid

#50837

Purpose

Fluorescent reporter for membrane PIP3 levels

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PHAkt (AKT1 )

Use

Lentiviral and Synthetic Biology

Tags

Cerulean

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-GINIP-Nluc W139A

Plasmid

#223557

Purpose

Plasmid expressing GINIP-Nluc W139A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

human GINIP W139A - linker(GGGS) - Nluc

Tags

3xFLAG

Expression

Mammalian

Mutation

W139A mutation in GINIP sequence

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAL149

Plasmid

#22275

Purpose

Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PhyB(1-908) (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

mCherry-Kras4BCT (prenylation sequence)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pLX304-VHL-IRES-Tdtomato

Plasmid

#187643

Purpose

VHL Expression vector with Tdtomato

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

VHL (VHL Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_SEPT9 i4

Plasmid

#71617

Purpose

mammalian expression of human SEPT9 i4 with a GFP fusion

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag JIP3b

Plasmid

#53458

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 JIP3a

Plasmid

#53457

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

T7

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
pHR SFFVp BFP-Erk2

Plasmid

#50848

Purpose

Fluorescent reporter for Erk nuclear translocation

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

TagBFP

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAL204

Plasmid

#22282

Purpose

Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBRed-d2-hygro

Plasmid

#139196

Purpose

non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBRed

Use

Luciferase and Retroviral

Tags

d2 PEST domain and nuclear localization signal (N…

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Tlr4

Plasmid

#27148

Depositor

Bruce Beutler

Article

Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

Insert

toll-like receptor 4 (Tlr4 Mouse)

Tags

3x FLAG

Expression

Mammalian

Mutation

Native signal peptide sequence (original amino ac…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C2_SEPT9 i1

Plasmid

#71609

Purpose

mammalian expression of human SEPT9 i1 with a GFP fusion.

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

Plasmid

#164184

Purpose

Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

Depositor

Thomas Oertner

Article

Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

Inserts

CamPARI2
Human α Tubulin

Use

AAV

Tags

6 His tag and NES

Expression

Mammalian

Promoter

Syn

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Tags

alkaline phosphatase and flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-myrAkt

Plasmid

#49267

Purpose

retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

Depositor

Lawrence Kane, Arthur Weiss

Article

Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

Insert

Akt (AKT1 Bovine)

Use

Retroviral

Tags

IRES-EGFP and myr

Expression

Mammalian

Mutation

N2D, D149E and A478G compared to AAW71957.1

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
mEos3.2GPI

Plasmid

#203759

Purpose

Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

CD58 c-term (CD58 Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF3

Plasmid

#207815

Purpose

N-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF3 (NFE2L3 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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