We narrowed to 1,546 results for: pbluescript

1. pCRISPaint-T2A-Gal4-3xP3-RFP

   Plasmid

   #127556

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK453 (BSD-P2A-dTAG-mCherry)

   Plasmid

   #214383

   Purpose

   dTAG-mCherry for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-dTAG-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWKS9

   Plasmid

   #139416

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTurquoise2
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACT5FVMF(f)

   Plasmid

   #110549

   Purpose

   Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

   Depositor

   David Russell

   Article

   Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

   Insert

   EGFP, FV LTRs
   

   Use

   Qpcr control for titering foamy virus

   Promoter

   promoter: MLV LTR

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mouse ZP3 promoter (JD#445)

   Plasmid

   #14634

   Depositor

   Jurrien Dean

   Article

   Rankin et al Development. 1998 Jul . 125(13):2415-24.

   Insert

   ZP3 promoter (Zp3 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK281 (mCherry2-Hygro)

   Plasmid

   #72797

   Purpose

   C-terminal tagging with mCherry2 using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mCherry2

   Expression

   Mammalian

   Available Since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2HLacZpA1I.7 (#59)

   Plasmid

   #35965

   Depositor

   Thomas Sato

   Article

   Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

   Inserts

   Tie2 promoter (Tek Mouse)
   SDK LacZ
   SV40 pA
   Tie2 full enhancer
   

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAID1.2-EF1a-NmScarlet-mAID

   Plasmid

   #140617

   Purpose

   Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

   Plasmid

   #52549

   Purpose

   transgenic construct for expression of GFP and Flpo

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   Flpo recombinase
   

   Expression

   Mammalian

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAID1.2-EF1a-NGFP-mAID

    Plasmid

    #140607

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK279 (mClover-Bsr)

    Plasmid

    #72795

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Bsr
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK278 (mClover-Hygro)

    Plasmid

    #72794

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Hygro
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS NL4-3 envFS eGFP PR-D25A

    Plasmid

    #101334

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Mutation

    Protease D25A

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSKB-

    Plasmid

    #62540

    Purpose

    Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDctp-mSox10-Mp1

    Plasmid

    #13513

    Depositor

    William Pavan

    Article

    Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

    Insert

    Sox10 (Sox10 Mouse)
    

    Tags

    Dct promoter and Mp1-polyA

    Expression

    Mammalian

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAID1.2-EF1a-NmCherry-mAID

    Plasmid

    #140603

    Purpose

    Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pdgfra-T2A-H2B-eGFP donor

    Plasmid

    #113120

    Purpose

    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWKS7

    Plasmid

    #139414

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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