161,152 results

1. pEGFP-C1-Nup35

   Plasmid

   #87342

   Purpose

   To express human Nup35 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   nucleoporin 35 (NUP35 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214506

   Purpose

   AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-Puro-sgNUAK2

   Plasmid

   #138693

   Purpose

   Expresses a human NUAK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK2 human (NUAK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF112

   Plasmid

   #135920

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Luciferase; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Neo-Bam p53 V143A

   Plasmid

   #16435

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 V143A (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V143A. Mutant cDNA from human colorectal tumor CX…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

6. HA-cdc20

   Plasmid

   #11594

   Depositor

   Marc Kirschner

   Article

   Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

   Insert

   cdc20 (CDC20 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-Mito-7

   Plasmid

   #54160

   Purpose

   Localization: Mitochondria, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AICSDP-4:TUBA1B-mEGFP

   Plasmid

   #87421

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pHyg-AID*-6HA

   Plasmid

   #99520

   Purpose

   C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   6xHA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO HIS HSPA8

    Plasmid

    #19541

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. WT NFAT1

    Plasmid

    #11100

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-Cas9-EGFP

    Plasmid

    #159126

    Purpose

    Expresses active Cas9 fused with EGFP for fluorescence microscopy

    Depositor

    Taekjip Ha

    Article

    Liu et al Science. 2020 Jun 12;368(6496):1265-1269

    Insert

    Cas9 fused to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. R5 PV

    Plasmid

    #51734

    Purpose

    Dicistronic vector with PV IRES

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    PV IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid

    #197028

    Purpose

    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D52R / T132R

    Promoter

    MHCK7, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD112

    Plasmid

    #119893

    Purpose

    pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-EF1afull-H2B-mCherry-P2A-Fluc

    Plasmid

    #207253

    Purpose

    Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    H2B-mCherry-P2A-Fluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha_full

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBSedit Sham

    Plasmid

    #223200

    Purpose

    Group B Streptococcus Efficient Genome Editing with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge