163,394 results

1. Flag-FOXN1

   Plasmid

   #153137

   Purpose

   Expression of Flag tagged human FOXN1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXN1 (FOXN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR Grb2-TagBFP

   Plasmid

   #188631

   Purpose

   Human Grb2 C-terminally labeled with TagBFP

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   GRB2 (GRB2 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIR-FbALFA

   Plasmid

   #184678

   Purpose

   NIR-Fb to ALFA-tag

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbALFA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-Foxo1 D256

   Plasmid

   #12145

   Depositor

   Domenico Accili

   Article

   Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

   Insert

   Foxo1 (Foxo1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a deletion truncating the protein after …

   Promoter

   CMV

   Available since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. TALED_Right-ND1-AD

   Plasmid

   #183900

   Purpose

   Expresses TALED_Right-ND1-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro CAG J591-scFV T-CAR

   Plasmid

   #171961

   Purpose

   Donor plasmid for J591 T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic J591-scFV CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. shYAP1 # 2

   Plasmid

   #42541

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-pDisplay-SNAP

    Plasmid

    #223495

    Purpose

    Expression of SNAP-tags to outer surface in mammalian cells

    Depositor

    James Frank

    Article

    Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

    Insert

    SNAP-tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLAMP1-miRFP670nano

    Plasmid

    #127435

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LAMP1-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-Neo-PIP-NLS-mVenus

    Plasmid

    #118619

    Purpose

    Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-NLS-mVenus
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISce1-D2EGFP

    Plasmid

    #32674

    Depositor

    Michael Tsang

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. dGFP-Rab27a

    Plasmid

    #170862

    Purpose

    Mammalian expression of dGFP-Rab27a.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Rab-27a (RAB27A Human)
    

    Use

    Tags

    dEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAKgfplux1

    Plasmid

    #14083

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PyronicSF/pcDNA3.1(-)

    Plasmid

    #124812

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. VIPR2-Tango

    Plasmid

    #66521

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    VIPR2 (VIPR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. EphA2-MT

    Plasmid

    #108851

    Purpose

    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Tags

    Labeled with mTurquoise on the C-Terminus via a 1…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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