165,688 results

1. pET empty polycistronic destination vector (2E)

   Plasmid

   #29775

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDUET-1_VNp-anti-6xHis mAb

   Plasmid

   #214952

   Purpose

   Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-6xHis mAb heavy chain
   VNp-anti-6xHis mAb light chain
   

   Tags

   VNp6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_MYCN_P2A_Hygro_Barcode

   Plasmid

   #120463

   Purpose

   Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCP-GFP

   Plasmid

   #101161

   Purpose

   to epress PCP-GFP fusion protein

   Depositor

   Mazhar Adli

   Article

   Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

   Insert

   PCP-GFP
   

   Use

   Lentiviral

   Promoter

   Ubc

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti PGK V5-LUC Neo (w623-2)

   Plasmid

   #21471

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   V5-LUC
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMP220

   Plasmid

   #185921

   Purpose

   Broad host range IncP promoter probe using lacZ

   Depositor

   Herman Spaink

   Article

   Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

   Type

   Empty backbone

   Use

   Incp

   Tags

   LacZ

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Myc-GRM1-IRES-CyRFP1

   Plasmid

   #195355

   Purpose

   mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

   Depositor

   Brenda Bloodgood

   Article

   Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

   Insert

   GRM1-IRES-CyRFP1
   

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-H3.3-WT-FLAG-BFP

   Plasmid

   #206139

   Purpose

   Expression of a FLAG tagged human histone H3.3 gene with BFP

   Depositor

   Maria Castro

   Insert

   H3.3 (H3-3A Human)
   

   Tags

   FLAG-eBFP

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-GCaMP6s-CAAX

   Plasmid

   #52228

   Purpose

   Plasma membrane targeted GCaMP6s, calcium sensor

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   GCaMP6s-CAAX
   

   Tags

   6xHis and CAAX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBIS

    Plasmid

    #17817

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-opto-mCasp1

    Plasmid

    #208781

    Purpose

    Inducibly expresses mouse caspase1 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase1 Cry2 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry, Cry2

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-Sec23A

    Plasmid

    #166894

    Purpose

    mammalian expression of Sec23A tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    SEC23A (SEC23A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. negMaLionG

    Plasmid

    #113907

    Purpose

    negative control for Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionG
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. 3541 pMIG Bcl-XL

    Plasmid

    #8790

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHis-hTG2

    Plasmid

    #100719

    Purpose

    Expression of human transglutaminase 2 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

    Insert

    TGM2 (TGM2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    silent mutation Arg580 (AGG--CGT) to enhance expr…

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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