We narrowed to 14,255 results for: TIM

Showing: 9941 - 9960 of 14255 results

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pYZ959

Plasmid

#192603

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

ScTEF1

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET302-6His-dCas9-Halo

Plasmid

#72269

Purpose

Encodes a Halo-labeled fusion of nuclease deficient Cas9

Depositor

Timothée Lionnet

Article

Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

Insert

His6-dCas9-Halo

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBudORF1-CH

Plasmid

#51290

Purpose

expresses the codon optimized ORF1 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1 ORF1 (L1RE1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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DYRK1A

Plasmid

#38913

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DYRK1A (DYRK1A Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pHCMV_WNV_PrME

Plasmid

#207313

Purpose

Mammalian expression of West Nile Virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

WNV_PrME (POLY West Nile Virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

Plasmid

#214926

Purpose

Expresses presynaptic non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).cpsfGFP.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI3

Plasmid

#42527

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Mutation

gene sequence is codon optimized for expression i…

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
Integrin alpha L - mCFP

Plasmid

#8637

Depositor

Timothy Springer

Article

Kim et al Science 2003 Sep 19;301(5640):1720-5.

Insert

Integrin, alpha L (ITGAL Human)

Tags

CFP

Expression

Mammalian

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pBudORF2-CH

Plasmid

#51289

Purpose

expresses the codon optimized ORF2 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 ORF2 (L1RE1 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMV_RVFV_GP

Plasmid

#207304

Purpose

Mammalian expression of Rift valley fever virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_JEV_prME

Plasmid

#207283

Purpose

Mammalian expression of Japanese encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

JEV_PrME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKm-HGBE

Plasmid

#170334

Purpose

a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized GBEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHIKV_E1E2

Plasmid

#207261

Purpose

Mammalian expression of Chikungunya virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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