We narrowed to 11,805 results for: nsf

1. AAV-TRE-NS1-dTomato

   Plasmid

   #175278

   Purpose

   AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   NS1; dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   dTomato (from bidirectional promoter)

   Promoter

   bidirectional TRE promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hUbC-dCas9-dMQ1-EGFG

   Plasmid

   #89794

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   hUbC

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.SF-iGluSnFR.S72A

   Plasmid

   #106200

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   GltI: S72A

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

   Plasmid

   #113693

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 gRNA Cassete
   

   Use

   AAV and CRISPR

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-flex-GFP-shRNA-GluN1

   Plasmid

   #182502

   Purpose

   Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Expression

   Mammalian

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

   Plasmid

   #110055

   Purpose

   Cry1 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Cry1 promoter and Venus (Cry1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Promoter

   Cry1

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn1-GRAB-HTR6-cilia

   Plasmid

   #189615

   Purpose

   Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6_GRAB
   

   Use

   AAV

   Promoter

   Syn1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-DIO-BiPOLES-mCerulean

   Plasmid

   #154949

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.SF-iGluSnFR.A184S

   Plasmid

   #106192

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_AR-RE_MLPmin_BC1014-luc2

    Plasmid

    #227108

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1014

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

    Plasmid

    #113679

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_CMV_CCR6-Nanoluc-V5

    Plasmid

    #199672

    Purpose

    expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    CCR6 (CCR6 Human)
    

    Use

    AAV

    Tags

    NanoLuc and V5

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-DIO-BiPOLES-mCerulean

    Plasmid

    #154950

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-ITR-PytR(A2.1)-mCherry

    Plasmid

    #204869

    Purpose

    Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. mazei pyrrolysyl tRNA mutant "A2.1"
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    U25C; A3G, A4G, T65C, T66C, T67T

    Promoter

    U6

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-SARS-CoV-2-3CL-C145A

    Plasmid

    #160279

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid

    #35509

    Purpose

    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV5 and AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

    Plasmid

    #78607

    Purpose

    A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    EF1a-short; U6

    Available Since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only